Rapid DNA purification for Hal gene PCR diagnosis in porcine tissues and extension to other meat species

Two different halothane (Hal) gene polymerase chain reaction (PCR) tests were applied to genomic DNA extracted from por cine blood, semen, muscle and fat tissues by a rapid and simple Chelex-100 based method. One of the PCR procedure is designed from the ryanodine receptor coding sequence to produce a 81 base pair (bp) fragment, while the other is designed from pig intron sequences to produce a 659 bp fragment. Oligonucleotide primers derived from the coding sequence were also used for other meat species. Amplification products obtained from porcine, bovine, ovine, equine and deer genomic DNA were successfully digested with Hha I restriction enzyme to produce the same electrophoretic pattern as in the normal homozygous (NN) pig. No PCR products could be amplified from chicken and turkey DNA. Copyright (C) 1996 Elsevier Science Ltd.