A High-Throughput Multiplex Method Adapted for GMO Detection

被引：50

作者：

Chaouachi, Maher ^{[1
,2
]}

Chupeau, Gaelle ^{[1
]}

Berapd, Aurelie ^{[1
]}

McKhann, Heather ^{[1
]}

Romaniuk, Marcel ^{[2
]}

Giancola, Sandra ^{[1
]}

Laval, Valerie ^{[2
]}

Bertheau, Yves ^{[2
]}

Brunel, Dominique ^{[1
]}

机构：

[1] INRA, CEA, Inst Genom, Ctr Natl Genotypage,UR EPGV, F-91057 Evry, France

[2] INRA, Lab Methodol Detect OGM, Unite PMDV, F-78026 Versailles, France

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2008年 / 56卷 / 24期

关键词：

SNPlex; GMO; detection; multiplex; high-throughput;

D O I：

10.1021/jf801482r

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A high-throughput multiplex assay for the detection of genetically modified organisms (GMO) was developed on the basis of the existing SNPlex method designed for SNP genotyping. This SNPlex assay allows the simultaneous detection of up to 48 short DNA sequences (similar to 70 bp; "signature sequences") from taxa endogenous reference genes, from GMO constructions, screening targets, construct-specific, and event-specific targets, and finally from donor organisms. This assay avoids certain shortcomings of multiplex PCR-based methods already in widespread use for GMO detection. The assay demonstrated high specificity and sensitivity. The results suggest that this assay is reliable, flexible, and cost- and time-effective for high-throughput GMO detection.

引用

页码：11596 / 11606

页数：11

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