Active immunization with cocaine-protein conjugate attenuates cocaine effects

被引：39

作者：

Ettinger, RH ^{[1
]}

Ettinger, WF ^{[1
]}

Harless, WE ^{[1
]}

机构：

[1] GONZAGA UNIV,DEPT BIOL,SPOKANE,WA 99258

来源：

PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR | 1997年 / 58卷 / 01期

关键词：

cocaine; antibodies; analgesia; reinforcement; HSAB; rats;

D O I：

10.1016/S0091-3057(97)00005-1

中图分类号：

B84 [心理学]; C [社会科学总论]; Q98 [人类学];

学科分类号：

03 ; 0303 ; 030303 ; 04 ; 0402 ;

摘要：

Immunization with cocaine-keyhole limpet hemocyanin (KLH) conjugate elicited the formation of anti-cocaine antibody sufficient to blunt the effects of cocaine in rats. Cocaine was bound to KLH for immunization with the photoactivatable crosslinker N-hydroxysuccinimide-4-azidobezoate (HSAB). Immunization with the cocaine-KLH-complete Freund's adjuvant complex was effective in attenuating the analgesic and reinforcing effects of cocaine in laboratory rats. Enzyme-linked dot blot assay revealed the presence of anti-cocaine antibody in serum. Competitive binding studies suggest that the antibody was specific to cocaine. Active immunization for cocaine may provide an alternative to drug treatment and may provide protection from addiction. (C) 1997 Elsevier Science Inc.

引用

页码：215 / 220

页数：6

共 23 条

[1]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[2] A POTENTIAL VACCINE FOR COCAINE ABUSE PROPHYLAXIS [J].

BAGASRA, O ;

FORMAN, LJ ;

HOWEEDY, A ;

WHITTLE, P .

IMMUNOPHARMACOLOGY, 1992, 23 (03) :173-179

[3]

BAGASRA O, 1994, IMMUNOPHARMACOLOGY, V27, P82, DOI 10.1016/0162-3109(94)90010-8

[4]

BLAKE MS, 1984, ANAL BIOCHEM, V1, P3

[5] SUPPRESSION OF PSYCHOACTIVE EFFECTS OF COCAINE BY ACTIVE IMMUNIZATION [J].

CARRERA, MRA ;

ASHLEY, JA ;

PARSONS, LH ;

WIRSCHING, P ;

KOOB, GF ;

JANDA, KD .

NATURE, 1995, 378 (6558) :727-730

[6]

CHRISTENSON JG, 1978, Patent No. 4102979

[7]

CUNNINGHAM KA, 1990, NEUROPSYCHOPHARMACOL, V3, P41

[8] A RADIOIMMUNOASSAY FOR METOCLOPRAMIDE [J].

DEVILLIERS, M ;

PARKIN, D ;

VANJAARSVELD, P ;

VANDERWALT, B .

JOURNAL OF IMMUNOLOGICAL METHODS, 1987, 103 (01) :33-39

[9]

GALLACHER G, 1994, IMMUNOPHARMACOLOGY, V27, P79, DOI 10.1016/0162-3109(94)90009-4

[10] COCAINE ADDICTION - PSYCHOLOGY AND NEUROPHYSIOLOGY [J].

GAWIN, FH .

SCIENCE, 1991, 251 (5001) :1580-1586

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