Modulation of immunologic reactions between cultured porcine hepatocytes and human sera

被引:12
作者
Hasegawa, H
Shimada, M
Gion, T
Ijima, H
Nakazawa, K
Funatsu, K
Sugimachi, K
机构
[1] Kyushu Univ, Fac Med, Dept Surg 2, Higashi Ku, Fukuoka 8128582, Japan
[2] Kyushu Univ, Fac Chem Engn, Dept Chem Engn, Higashi Ku, Fukuoka 8128582, Japan
关键词
D O I
10.1097/00002480-199909000-00005
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
In the clinical application of a hybrid artificial liver system using porcine hepatocytes, some immunologic reactions occur between human serum and porcine hepatocytes. In this study, we investigated the immunologic mechanisms of the cytotoxic reactions, and we tried to inactivate the human serum cytotoxicity by heating the serum or the addition of nafamostat mesilate; (NM), Immunologic reaction between human serum and porcine hepatocytes by evaluating the immunochemical response against human IgM, IgG, and C3 was investigated. The immunochemical analysis of inactivation by heated human serum (56 degrees C, 30 min) and adding NM were performed. The evaluation of serum cytotoxicity was as follows: when porcine hepatocytes were cultured with heating the human serum or the addition of NM, the survival ratio was observed, Immunochemical reactions against human C3 were all positive, but positive reaction against human IgM occurred in only one case (5%); those against human Ige were all negative. Both heating the serum and adding NM inhibited the immunochemical reaction of human C3. The inhibition of human C3 with NM was dependent on that concentration. Both heating of the serum and adding NM to the medium decreased damage of porcine hepatocytes. An immunologic reaction between human serum and porcine hepatocytes in a porcine bioartificial liver clearly occurred, and this reaction was controlled by heating the serum and adding NM. We believe that NM is useful in the clinical application of our hybrid artificial liver system.
引用
收藏
页码:392 / 396
页数:5
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