Counting integral numbers of gamma-carboxyglutamic acids per peptide chain using capillary electrophoresis

被引:3
作者
Black, RS
Adams, GM
Jorgenson, JW
Hiskey, RG
机构
[1] Department of Chemistry, Univ. N. Carolina Chapel Hill C., Venable Hall, Chapel Hill
基金
美国国家卫生研究院; 美国国家科学基金会;
关键词
D O I
10.1080/10826079708005560
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method for counting the number of gamma-carboxyglutamic acid (Gla) residues in peptides is presented. The product mixture generated by partial decarboxylation of a peptide containing multiple Gla residues is separated by capillary electrophoresis. A mixture derived from a peptide containing n Gla residues produces an electropherogram characterized by n + 1 peak groups. For a given peptide, a linear relationship r(2) > 0.99) is observed between the average electrophoretic mobility of each peak group and the representative number of Gla residues contained by that group. Resolution of peptide isomers is also obtained, permitting studies of the extent and sequence specificity of decarboxylation.
引用
收藏
页码:2009 / 2021
页数:13
相关论文
共 30 条
[1]  
ADAMS GM, 1992, THESIS U N CAROLINA
[2]   ESSENTIAL PEAK AREA NORMALIZATION FOR QUANTITATIVE IMPURITY CONTENT DETERMINATION BY CAPILLARY ELECTROPHORESIS [J].
ALTRIA, KD .
CHROMATOGRAPHIA, 1993, 35 (3-4) :177-182
[3]  
BAJAJ SP, 1982, J BIOL CHEM, V257, P3726
[4]   MULTI-SITE-SPECIFICITY OF THE VITAMIN-K-DEPENDENT CARBOXYLASE - IN-VITRO CARBOXYLATION OF DES-GAMMA-CARBOXYLATED BONE GLA PROTEIN AND DES-GAMMA-CARBOXYLATED PRO BONE GLA PROTEIN [J].
BENTON, ME ;
PRICE, PA ;
SUTTIE, JW .
BIOCHEMISTRY, 1995, 34 (29) :9541-9551
[5]  
BOROWSKI M, 1986, J BIOL CHEM, V261, P1624
[6]   COUNTING INTEGRAL NUMBERS OF AMINO-ACID-RESIDUES PER POLYPEPTIDE-CHAIN [J].
CREIGHTON, TE .
NATURE, 1980, 284 (5755) :487-489
[7]   THE COAGULATION CASCADE - INITIATION, MAINTENANCE, AND REGULATION [J].
DAVIE, EW ;
FUJIKAWA, K ;
KISIEL, W .
BIOCHEMISTRY, 1991, 30 (43) :10363-10370
[8]  
DECOTTIGNIESLEMARECHAL P, 1979, BIOCHEM BIOPH RES CO, V90, P700
[9]   MECHANISM OF ACTION OF VITAMIN-K [J].
DOWD, P ;
HAM, SW ;
GEIB, SJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (20) :7734-7743
[10]   SIDE-CHAIN INTERACTIONS BETWEEN ARGININE AND GAMMA-CARBOXYGLUTAMIC ACID - STUDIES ON DECARBOXYLATION OF GAMMA-CARBOXYGLUTAMIC ACID IN THE PRESENCE OF GUANIDINE [J].
GRAY, AL ;
HOKE, RA ;
DEERFIELD, DW ;
HISKEY, RG .
JOURNAL OF ORGANIC CHEMISTRY, 1985, 50 (12) :2189-2191