Glycolytic-methylglyoxal pathway -: Molecular evolution and stress response of glyoxalase I in Saccharomyces cerevisiae

被引：14

作者：

Inoue, Y ^{[1
]}

Kimura, A ^{[1
]}

机构：

[1] Kyoto Univ, Food Sci Res Inst, Kyoto 6110011, Japan

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 1999年 / 75卷 / 06期

关键词：

glyoxalase I; methylglyoxal; Saccharomyces cerevisiae; gene duplication; molecular evolution; stress response; MAP kinase; glutathione;

D O I：

10.2183/pjab.75.127

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Methylglyoxal is an endogenous cytotoxic compound formed as a byproduct of glycolysis. We systematically analyzed the metabolic fate of methylglyoxal in various microorganisms and found that glyoxalase I is a ubiquitous and critical enzyme for its detoxification. We found that glyoxalase I consists of five segments (regions I-V) which are conserved among the glyoxalase Is of various species. We hypothesize that yeast glyoxalase I evolved by gene duplication and here show supporting evidence. We also found that expression of the structural gene for the glyoxalase I of S. cerevisiae is induced by osmotic stress through the HOG-MAG kinase signaling pathway. We identified the physiological significance of this glycolytic-methylglyoxal pathway in S, cerevisiae.

引用

页码：127 / 132

页数：6

共 29 条

[1] GPD1, WHICH ENCODES GLYCEROL-3-PHOSPHATE DEHYDROGENASE, IS ESSENTIAL FOR GROWTH UNDER OSMOTIC-STRESS IN SACCHAROMYCES-CEREVISIAE, AND ITS EXPRESSION IS REGULATED BY THE HIGH-OSMOLARITY GLYCEROL RESPONSE PATHWAY [J].