A poxvirus-like type IB topoisomerase family in bacteria

We report that diverse species of bacteria encode a type 113 DNA topoisomerase that resembles vaccinia virus topoisomerase. Deinococcus radiodurans topoisomerase 113 (DraTopIB), an exemplary member of this family, relaxes supercoiled DNA in the absence of a divalent cation or ATP. DraTopIB has a compact size (346 aa) and is a monomer in solution. Mutational analysis shows that the active site of aDraTopIB is composed of the same constellation of catalytic side chains as the vaccinia enzyme. Sequence comparisons and limited proteolysis suggest that their folds are conserved. These findings imply an intimate evolutionary relationship between the poxvirus and bacterial type 1B enzymes, and they engender a scheme for the evolution of topoisomerase 1B and tyrosine recombinases from a common ancestral strand transferase in the bacterial domain. Remarkably, bacteria that possess topoisomerase 1B appear to lack DNA topoisomerase III.