Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression

被引：93

作者：

Cluster, PD ^{[1
]}

ODell, M ^{[1
]}

Metzlaff, M ^{[1
]}

Flavell, RB ^{[1
]}

机构：

[1] JOHN INNES CTR PLANT SCI RES,NORWICH NR4 7UH,NORFOLK,ENGLAND

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 32卷 / 06期

关键词：

Agrobacterium-mediated transformation; binary-verector transfer; direct repeats; inverted repeats; repeat-induced gene silencing; transgene inheritance;

D O I：

10.1007/BF00041406

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Analysis of Agrobacterium-transferred DNA (T-DNA) revealed strong correlations between transgene structures and floral pigmentation patterns from chalcone synthase (chs) co-suppression among 47 Petunia transformants. Presented here are the full details of T-DNA structural organization in that population. Sixteen transformants (34%) carried one T-DNA copy while 31 (66%) carried 106 complete and partial T-DNA elements in 54 linkage groups. Thirty linkage groups contained multiple T-DNA copies; 15 of these contained only contiguously repeated copies, 8 contained only dispersed copies and 7 contained both. Right-border inverted repeats were three times more frequent than left-border inverted or direct repeats. Large fragments of binary-vector sequences were linked to the T-DNA in seven plants.

引用

页码：1197 / 1203

页数：7

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