Production of a monoclonal antibody directed against the high-affinity nerve growth factor receptor

被引：6

作者：

Tagliabue, E ^{[1
]}

Ghirelli, C ^{[1
]}

Lombardi, L ^{[1
]}

Castiglioni, F ^{[1
]}

Asnaghi, L ^{[1
]}

Longhi, C ^{[1
]}

Borrello, MG ^{[1
]}

Aiello, P ^{[1
]}

Ménard, S ^{[1
]}

机构：

[1] Ist Nazl Tumori, Dept Expt Oncol, Mol Targeting Unit, I-20133 Milan, Italy

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS | 1999年 / 14卷 / 02期

关键词：

high-affinity nerve growth factor receptor monoclonal antibody; somatic fusion;

D O I：

10.1177/172460089901400203

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The high-affinity nerve growth factor receptor corresponds to the tyrosine protein kinase encoded by the proto-oncogene trkA. Different findings suggest that nerve growth factor (NGF) can be operative in the growth modulation of tumor cell lines possessing high-affinity binding sites for this molecule. Using as immunizing material the SKNBE neuroblastoma cell line transfected with proto-trkA we produced a monoclonal antibody (MAb) able to recognize the high-affinity nerve growth factor receptor. The selected MAb, designated MGR12, is directed against an epitope present on the extracellular domain of the receptor since it showed reactivity on living trkA-expressing cells and was able to immunoprecipitate the proto-trkA molecule. The MGR12 MAb is directed against a non-functional epitope since it neither inhibited NGF binding nor induced receptor internalization. This new reagent appears to be an appropriate tool for analyzing the expression of high-affinity nerve growth factor receptor in tumors of different origin and for elucidating its involvement in tumor progression.

引用

页码：68 / 72

页数：5

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