Two-metal active site binding of a Tn5 transposase synaptic complex

被引：65

作者：

Lovell, S ^{[1
]}

Goryshin, IY ^{[1
]}

Reznikoff, WR ^{[1
]}

Rayment, I ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biochem, Madison, WI 53706 USA

来源：

NATURE STRUCTURAL BIOLOGY | 2002年 / 9卷 / 04期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1038/nsb778

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A synaptic complex of Tn 5 transposase with an extended outside end DNA duplex was prepared and crystallized, and its crystal structure was determined in an effort to reveal the role of metal ions in catalysis. Two Mn2+ ions bound to the active site when a single nucleotide of donor DNA was added to the 3' end of the transferred strand. Marked conformational changes were observed in the DNA bases closest to the active site. The position of the metal ions and the conformational changes of the DNA provide insight into the mechanism of hairpin formation and cleavage, and is consistent with a two-metal model for catalysis.

引用

收藏

页码：278 / 281

页数：4

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