A reverse transcription-polymerase chain reaction assay for the detection of avian pneumovirus (Colorado strain)

被引：11

作者：

Ali, A ^{[1
]}

Reynolds, DL ^{[1
]}

机构：

[1] Iowa State Univ Sci & Technol, Coll Vet Med, Dept Vet Microbiol & Prevent Med, Vet Med Res Inst, Ames, IA 50011 USA

来源：

AVIAN DISEASES | 1999年 / 43卷 / 03期

关键词：

turkey rhinotracheitis; avian pneumovirus; RT-PCR;

D O I：

10.2307/1592663

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A reverse transcription-polymerase chain reaction assay was developed for the detection of avian pneumovirus (Colorado strain) (APV-Col). The specific primers were designed from the published sequence of the matrix protein gene of APV-Col. The primers amplified a product of 631 nucleotides from APV-Col. The assay identified only APV-Col and did not react with Newcastle disease virus and infectious bronchitis virus.

引用

页码：600 / 603

页数：4

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