Evaluation of CD4+CD25+FOXP3+ regulatory T cells function in patients with common variable immunodeficiency

被引：54

作者：

Arandi, Nargess ^{[1
]}

Mirshafiey, Abbas ^{[1
]}

Jeddi-Tehrani, Mahmood ^{[2
]}

Abolhassani, Hassan ^{[3
]}

Sadeghi, Bamdad ^{[3
]}

Mirminachi, Babak ^{[3
]}

Shaghaghi, Mohammadreza ^{[3
]}

Aghamohammadi, Asghar ^{[3
]}

机构：

[1] Univ Tehran Med Sci, Sch Publ Hlth, Dept Immunol, Tehran, Iran

[2] ACECR, Avicenna Res Inst, Monoclonal Antibody Res Ctr, Tehran, Iran

[3] Univ Tehran Med Sci, Childrens Med Ctr, Pediat Ctr Excellence, Res Ctr Immunodeficiencies, Tehran, Iran

来源：

CELLULAR IMMUNOLOGY | 2013年 / 281卷 / 02期

关键词：

Common variable immunodeficiency (CVID); Regulatory T cells; Suppressive function; IL-10; TGF-beta; MEMORY B-CELLS; IMMUNOLOGICAL FEATURES; FOXP3; DIFFERENTIATION; EXPRESSION; FREQUENCY; SUBSETS; IL-10;

D O I：

10.1016/j.cellimm.2013.03.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Common variable immunodeficiency (CVID) is one of the predominant antibody disorders where abnormalities in regulatory T cells (Tregs) may result in autoimmunity and chronic inflammation. To evaluate Tegs frequency and function, 13 CVID patients and 10 age- and sex-matched healthy volunteer were enrolled. The percentages of Tregs were calculated using flow cytomety method. For assessment of Treg function, Tregs were isolated and their suppressive functions were determined using Tregs suppression assay. The levels of immunoregulatory cytokines IL-10 and TGF-beta produced by Tregs were also measured. Our results revealed that Tregs frequency (P < 0.001) and their suppressive functions (P < 0.001) were impaired in CVID patients. The level of TGF-beta did not differ between CVID patients and controls (p = 0.09); while the amount of IL-10 was remarkably decreased in CVID patients (P = 0.007). Our findings suggest that disturbed Tregs frequency and their functional characteristics might account for aberrant immune responses observed in CVID patients. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：129 / 133

页数：5

共 33 条

[1]

Clinical and immunological features of 65 Iranian patients with common variable immunodeficiency [J].