Differential expression of c-fos and tyrosine hydroxylase mRNA in the adrenal gland of the infant rat:: Evidence for an adrenal hyporesponsive period

被引:40
作者
Okimoto, DK
Blaus, A
Schmidt, M
Gordon, MK
Dent, GW
Levine, S
机构
[1] Univ Calif Davis, Ctr Neurosci, Davis, CA 95616 USA
[2] Univ Delaware, Dept Biol Sci, Newark, DE 19716 USA
[3] Leiden Univ, Div Med Pharmacol, Leiden Amsterdam Ctr Drug Res, NL-2300 RA Leiden, Netherlands
[4] Univ Delaware, Dept Psychol, Newark, DE 19716 USA
[5] Bristol Myers Squibb Pharma Co, CNS Dis, Glenolden, PA 19036 USA
关键词
D O I
10.1210/en.143.5.1717
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Rats exhibit a stress hyporesponsive period from postnatal day (PND) 4-14 in which the neonate displays a minimal corticosterone response to stress. We used the maternal deprivation model to test whether this adrenocortical hyporesponsiveness to stress results from a decrease in adrenal sensitivity to ACTH. Neonates (PND 6,9, and 12) were injected ip with dexamethasone to block endogenous ACTH release, and 4 h later injected with graded doses of ACTH and killed. In another experiment, neonates were injected with isotonic saline and adrenal glands were collected at 30,60, and 120 min post injection to examine c-fos and tyrosine hydroxylase mRNA levels using in situ hybridization. Maternally deprived pups demonstrated elevated corticosterone levels at the two highest ACTH doses and showed a greater magnitude in glucocorticoid secretion compared with the nondeprived pups. Maternally deprived pups given a saline injection exhibited elevated basal and stress-induced levels of corticosterone, in contrast to the nondeprived pups that showed a minimal response. Strikingly, maternally deprived pups exhibited elevated levels of adrenocortical c-fos mRNA, whereas the nondeprived pups did not. In contrast, the pattern of c-fos gene expression in the adrenal medulla in both groups did not display any correlation with glucocorticoid secretion. Tyrosine hydroxylase gene expression in the adrenal medulla was observed in both nondeprived and maternally deprived pups, with the latter exhibiting an earlier response of greater magnitude. These results demonstrate that the suppression of steroidogenesis occurs directly in the adrenal cortex and provide further evidence for an adrenal hyporesponsive period in the rat.
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页码:1717 / 1725
页数:9
相关论文
共 54 条
[1]   Role of leptin in the neuroendocrine response to fasting [J].
Ahima, RS ;
Prabakaran, D ;
Mantzoros, C ;
Qu, DQ ;
Lowell, B ;
MaratosFlier, E ;
Flier, JS .
NATURE, 1996, 382 (6588) :250-252
[2]   Postnatal leptin surge and regulation of circadian rhythm of leptin by feeding - Implications for energy homeostasis and neuroendocrine function [J].
Ahima, RS ;
Prabakaran, D ;
Flier, JS .
JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (05) :1020-1027
[3]  
Ballard P L, 1979, Monogr Endocrinol, V12, P493
[4]   EFFECTS OF POSTNATAL RESERPINE ADMINISTRATION ON SYMPATHO-ADRENAL DEVELOPMENT IN RAT [J].
BARTOLOME, J ;
SLOTKIN, TA .
BIOCHEMICAL PHARMACOLOGY, 1976, 25 (13) :1513-1519
[5]  
BARTOLOME J, 1979, BIOCHEM PHARMACOL, V25, P2378
[6]  
Bohn M.C., 1984, NEUROBEHAVIORAL TERA, P365
[7]   Evidence for a novel peripheral action of leptin as a metabolic signal to the adrenal gland - Leptin inhibits cortisol release directly [J].
Bornstein, SR ;
Uhlmann, K ;
Haidan, A ;
EhrhartBornstein, M ;
Scherbaum, WA .
DIABETES, 1997, 46 (07) :1235-1238
[8]   NON-NEUROGENIC MECHANISM FOR RESERPINE-INDUCED RELEASE OF CATECHOLAMINES FROM THE ADRENAL-MEDULLA OF NEONATAL RATS - POSSIBLE MODULATION BY OPIATE RECEPTORS [J].
CHANTRY, CJ ;
SEIDLER, FJ ;
SLOTKIN, TA .
NEUROSCIENCE, 1982, 7 (03) :673-678
[9]  
CURRAN T, 1987, ONCOGENE, V2, P79
[10]   Stress-induced alterations in corticotropin-releasing hormone and vasopressin gene expression in the paraventricular nucleus during ontogeny [J].
Dent, GW ;
Okimoto, DK ;
Smith, MA ;
Levine, S .
NEUROENDOCRINOLOGY, 2000, 71 (06) :333-342