Removal of promoter nucleosomes by disassembly rather than sliding in vivo

被引：206

作者：

Boeger, H ^{[1
]}

Griesenbeck, J ^{[1
]}

Strattan, JS ^{[1
]}

Kornberg, RD ^{[1
]}

机构：

[1] Stanford Univ, Sch Med, Dept Biol Struct, Stanford, CA 94305 USA

来源：

MOLECULAR CELL | 2004年 / 14卷 / 05期

关键词：

D O I：

10.1016/j.molcel.2004.05.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previous work demonstrated the removal of nucleosomes from the PHO5 promoter upon transcriptional activation in yeast. Removal could occur by nucleosome disassembly or by sliding of nucleosomes away from the promoter. We have now activated the PHO5 promoter on chromatin circles following excision from the chromosomal locus. Whereas sliding would conserve the number of nucleosomes on the circle, we found that the number was diminished, demonstrating chromatin remodeling by nucleosome disassembly.

引用

页码：667 / 673

页数：7

共 29 条

[1] Functional differences between the human ATP-dependent nucleosome remodeling proteins BRG1 and SNF2H
Aalfs, JD
Narlikar, GJ
Kingston, RE
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (36) : 34270 - 34278
[2] The histone variant H3.3 marks active chromatin by replication-independent nucleosome assembly
Ahmad, K
Henikoff, S
[J]. MOLECULAR CELL, 2002, 9 (06) : 1191 - 1200
[3] NUCLEASE HYPERSENSITIVE REGIONS WITH ADJACENT POSITIONED NUCLEOSOMES MARK THE GENE BOUNDARIES OF THE PHO5/PHO3 LOCUS IN YEAST
ALMER, A
HORZ, W
[J]. EMBO JOURNAL, 1986, 5 (10) : 2681 - 2687
[4] Multiple mechanistically distinct functions of SAGA at the PH05 promoter
Barbaric, S
Reinke, H
Hörz, W
[J]. MOLECULAR AND CELLULAR BIOLOGY, 2003, 23 (10) : 3468 - 3476
[5] Nucleosomes unfold completely at a transcriptionally active promoter
Boeger, H
Griesenbeck, J
Strattan, JS
Kornberg, RD
[J]. MOLECULAR CELL, 2003, 11 (06) : 1587 - 1598
[6] CONFORMATIONAL FLUCTUATIONS OF DNA HELIX
DEPEW, RE
WANG, JC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (11) : 4275 - 4279
[7] Chromatin remodeling in vivo: Evidence for a nucleosome sliding mechanism
Fazzio, TG
Tsukiyama, T
[J]. MOLECULAR CELL, 2003, 12 (05) : 1333 - 1340
[8] RNA polymerase II holoenzyme recruitment is sufficient to remodel chromatin at the yeast PHO5 promoter
Gaudreau, L
Schmid, A
Blaschke, D
Ptashne, M
Horz, W
[J]. CELL, 1997, 89 (01) : 55 - 62
[9] FOLDING OF DNA DOUBLE HELIX IN CHROMATIN-LIKE STRUCTURES FROM SIMIAN VIRUS 40
GERMOND, JE
HIRT, B
OUDET, P
GROSSBELLARD, M
CHAMBON, P
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (05) : 1843 - 1847
[10] Absence of Gcn5 HAT activity defines a novel state in the opening of chromatin at the PHO5 promoter in yeast
Gregory, PD
Schmid, A
Zavari, M
Lui, L
Berger, SL
Horz, W
[J]. MOLECULAR CELL, 1998, 1 (04) : 495 - 505

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