Identification and expression of a factor of the DM family in the oyster Crassostrea gigas

被引：60

作者：

Naimi, Amine ^{[1
]}

Martinez, Anne-Sophie ^{[1
]}

Specq, Marie-Laure ^{[1
]}

Mrac, Abdellah ^{[1
]}

Diss, Blandine ^{[2
]}

Mathieu, Michel ^{[1
]}

Sourdaine, Pascal ^{[1
]}

机构：

[1] Univ Caen, IBFA, IFR ICORE 146, UMR 100 Ifremer Physiol & Ecophysiol Mollusques M, F-14032 Caen, France

[2] Soc Atlantique Mariculture, SATMAR, F-50760 La Saline, Gatteville Phar, France

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 2009年 / 152卷 / 02期

关键词：

Oyster; Crassostrea gigas; Dmrt1; Dmrt4; Dmrt5; Sex determination; SEXUALLY DIMORPHIC EXPRESSION; VASA-LIKE GENE; PACIFIC OYSTER; MOLECULAR-CLONING; DMRT1; SEX; GONADS; MEDAKA; GERMLINE; PROTEIN;

D O I：

10.1016/j.cbpa.2008.09.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Pacific oyster Crassostrea gigas is a successive not systematic protandric hermaphrodite. Searching for an ortholog to Dmrt1, a conserved sex determinism factor, we have identified the first complete cDNA of a DM factor in Lophotrochozoa which we have called Cg-DMl (Crassostrea gigas DMRT-like). It is 359aa long, with the DM domain common to all the family factors, and one DMA domain specific to members such as Dmrt4 and Dmrt5. Its gene presents one intron of 598 bp. Real time PCR and in situ hybridization have shown that Cg-DMl was expressed in both sexes, with a significantly higher expression in male than in female gonads at the end of the adult gametogenetic cycle and that a significant peak of expression was observed in spat between 1 and 2 months of age. These results suggest that Cg-DMl may be involved in the development of the gonad and may constitute preliminary clues for future work in order to better understand DM protein evolution. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：189 / 196

页数：8

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