Characterization of Dak Nong virus, an insect nidovirus isolated from Culex mosquitoes in Vietnam

被引：27

作者：

Kuwata, Ryusei ^{[1
]}

Satho, Tomomitsu ^{[2
]}

Isawa, Haruhiko ^{[1
]}

Nguyen Thi Yen ^{[3
]}

Tran Vu Phong ^{[3
]}

Phan Thi Nga ^{[4
]}

Kurashige, Tomokazu ^{[2
]}

Hiramatsu, Yukihiro ^{[2
]}

Fukumitsu, Yuki ^{[2
]}

Hoshino, Keita ^{[1
]}

Sasaki, Toshinori ^{[1
]}

Kobayashi, Mutsuo ^{[1
]}

Mizutani, Tetsuya ^{[5
]}

Sawabe, Kyoko ^{[1
]}

机构：

[1] Natl Inst Infect Dis, Dept Med Entomol, Shinjuku Ku, Tokyo 1628640, Japan

[2] Fukuoka Univ, Fac Pharmaceut Sci, Jonan Ku, Fukuoka 8140180, Japan

[3] Natl Inst Hyg & Epidemiol, Dept Med Entomol & Zool, Hanoi 100000, Vietnam

[4] Natl Inst Hyg & Epidemiol, Dept Training & Res Management, Hanoi 100000, Vietnam

[5] Tokyo Univ Agr & Technol, Res & Educ Ctr Prevent Global Infect Dis Anim, Fuchu, Tokyo 1838509, Japan

来源：

ARCHIVES OF VIROLOGY | 2013年 / 158卷 / 11期

关键词：

CORONAVIRUS SPIKE PROTEIN; FUNCTIONAL-CHARACTERIZATION; NUCLEOCAPSID PROTEIN; PREDICTION; CELL; FLAVIVIRUS; CLEAVAGE; EVOLUTION; REVEALS; DIPTERA;

D O I：

10.1007/s00705-013-1741-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study, we isolated and characterized an insect nidovirus from the mosquito Culex tritaeniorhynchus Giles (Diptera: Culicidae) in Vietnam, as an additional member of the new family Mesoniviridae in the order Nidovirales. The virus, designated "Dak Nong virus (DKNV)," shared many characteristics with Cavally virus and Nam Dinh virus, which have also been discovered recently in mosquitoes, and these viruses should be considered members of a single virus species, Alphamesonivirus 1. DKNV grew in cultured mosquito cells but could not replicate in the cultured vertebrate cells tested. N-terminal sequencing of the DKNV structural proteins revealed two posttranslational cleavage sites in the spike glycoprotein precursor. DKNV is assumed to be a new member of the species Alphamesonivirus 1, and the current study provides further understanding of viruses belonging to the new family Mesoniviridae.

引用

页码：2273 / 2284

页数：12

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