A radiation hybrid map of the RN region in pigs demonstrates conserved gene order compared with the human and mouse genomes

被引：16

作者：

Robic, A

Seroude, V

Jeon, JT

Yerle, M

Wasungu, L

Andersson, L

Gellin, J

Milan, D

机构：

[1] INRA, Lab Genet Cellulaire, F-31326 Castanet Tolosan, France

[2] Swedish Univ Agr Sci, Uppsala Biomed Ctr, Dept Anim Breeding & Genet, S-75124 Uppsala, Sweden

来源：

MAMMALIAN GENOME | 1999年 / 10卷 / 06期

关键词：

D O I：

10.1007/s003359901045

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recently constructed a 7000-rad porcine whole-genome radiation hybrid (RH) panel with the primary objective of integrating linkage maps of microsatellites with evolutionary conserved genes into one ordered map. In order to evaluate the resolution of this RH panel, we have now constructed a radiation hybrid map of the Chromosome (Chr) 15q2.3-q2.6 region containing the RN gene. This gene has large effects on glycogen content in muscle and meat quality. Ten microsatellites covering a region of 55 centiMorgans and eight genes (AE3, FN1, IGFBP5, INHA, IRS1, PAX3, TNP1, and VIL1) were placed on the Sscr15 RH map. All the genes, except IRS1, were mapped on the RH map between microsatellites located in 15q2.5. The relative order of AE3 and INHA was inverted on the porcine physical map in comparison with the mouse linkage map. The order of other genes already mapped in the mouse (FN1, IGFBP5, TNP1, VIL1, INHA/AE3, and PAX3) was identical in pigs. We found no clear difference between the gene order on pig Chr 15 and human Chr 2q.

引用

页码：565 / 568

页数：4

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