Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content

被引：158

作者：

Weselake, Randall J. ^{[2
]}

Shah, Saleh ^{[3
]}

Tang, Mingguo ^{[1
]}

Quant, Patti A. ^{[4
]}

Snyder, Crystal L. ^{[2
]}

Furukawa-Stoffer, Tara L. ^{[2
]}

Zhu, Weiming ^{[3
]}

Taylor, David C. ^{[5
]}

Zou, Jitao ^{[5
]}

Kumar, Arvind ^{[5
]}

Hall, Linda ^{[2
]}

Laroche, Andre ^{[6
]}

Rakow, Gerhard ^{[7
]}

Raney, Phillip ^{[7
]}

Moloney, Maurice M. ^{[8
]}

Harwood, John L. ^{[1
]}

机构：

[1] Cardiff Univ, Sch Biosci, Cardiff CF10 3AX, S Glam, Wales

[2] Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB T6G 2P5, Canada

[3] Alberta Res Council, Plant Biotechnol Unit, Vegreville, AB T9C 1T4, Canada

[4] Univ Oxford, Dept Biochem, Oxford OX1 3QU, England

[5] Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada

[6] Agr & Agri Food Canada, Lethbridge, AB T1J 4B1, Canada

[7] Agr & Agri Food Canada, Saskatoon, SK S7N 0X2, Canada

[8] Univ Calgary, Calgary, AB T2N 1N4, Canada

来源：

JOURNAL OF EXPERIMENTAL BOTANY | 2008年 / 59卷 / 13期

基金：

加拿大创新基金会; 加拿大自然科学与工程研究理事会;

关键词：

Brassica napus; control analysis; diacylglycerol acyltransferase; oil content; oilseed modification;

D O I：

10.1093/jxb/ern206

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Top-down control analysis (TDCA) is a useful tool for quantifying constraints on metabolic pathways that might be overcome by biotechnological approaches. Previous studies on lipid accumulation in oilseed rape have suggested that diacylglycerol acyltransferase (DGAT), which catalyses the final step in seed oil biosynthesis, might be an effective target for enhancing seed oil content. Here, increased seed oil content, increased DGAT activity, and reduced substrate:product ratio are demonstrated, as well as reduced flux control by complex lipid assembly, as determined by TDCA in Brassica napus (canola) lines which overexpress the gene encoding type-1 DGAT. Lines overexpressing DGAT1 also exhibited considerably enhanced seed oil content under drought conditions. These results support the use of TDCA in guiding the rational selection of molecular targets for oilseed modification. The most effective lines had a seed oil increase of 14%. Moreover, overexpression of DGAT1 under drought conditions reduced this environmental penalty on seed oil content.

引用

页码：3543 / 3549

页数：7

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