Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies

被引：236

作者：

Ehricht, R

Slickers, P

Goellner, S

Hotzel, H

Sachse, K

机构：

[1] Clondiag Chip Technol GmbH, D-07743 Jena, Germany

[2] Fed Res Inst Anim Hlth, Friedrich Loeffler Inst, Inst Bacterial Infect & Zoonoses, D-07743 Jena, Germany

来源：

MOLECULAR AND CELLULAR PROBES | 2006年 / 20卷 / 01期

关键词：

DNA microarray; sensitivity; detection; Chlamydia trachomatis;

D O I：

10.1016/j.mcp.2005.09.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

This study was conducted to determine the detection limit of an optimized DNA microarray assay for detection and species identification of chlamydiae. Examination of dilution series of a plasmid standard carrying the target sequence from Chlamydia trachomatis and genomic DNA of this organism revealed that a single PCR-amplifiable target copy was sufficient to obtain a specific hybridization pattern. This performance renders the test suitable for routine testing of clinical samples. (c) 2005 Elsevier Ltd. All rights reserved.

引用

收藏

页码：60 / 63

页数：4

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