mRNA-based parallel detection of active methanotroph populations by use of a diagnostic microarray

被引：62

作者：

Bodrossy, L ^{[1
]}

Stralis-Pavese, N

Konrad-Köszler, M

Weilharter, A

Reichenauer, TG

Schöfer, D

Sessitsch, A

机构：

[1] ARC Seibersdorf Res GmbH, Dept Bioresources, A-2444 Seibersdorf, Austria

[2] ARC Seibersdorf Res GmbH, Dept Environm Res, A-2444 Seibersdorf, Austria

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2006年 / 72卷 / 02期

关键词：

D O I：

10.1128/AEM.72.2.1672-1676.2006

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method was developed for the mRNA-based application of microbial diagnostic microarrays to detect active microbial populations. DNA- and mRNA-based analyses of environmental samples were compared and confirmed via quantitative PCR. Results indicated that mRNA-based microarray analyses may provide additional information on the composition and functioning of microbial communities.

引用

页码：1672 / 1676

页数：5

共 17 条

[1] The isotope array, a new tool that employs substrate-mediated labeling of rRNA for determination of microbial community structure and function [J].

Adamczyk, J ;

Hesselsoe, M ;

Iversen, N ;

Horn, M ;

Lehner, A ;

Nielsen, PH ;

Schloter, M ;

Roslev, P ;

Wagner, M .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (11) :6875-6887

[2] Ribosomal RNA-targeted nucleic acid probes for studies in microbial ecology [J].

Amann, R ;

Ludwig, W .

FEMS MICROBIOLOGY REVIEWS, 2000, 24 (05) :555-565

[3] Oligonucleotide microarrays in microbial diagnostics [J].

Bodrossy, L ;

Sessitsch, A .

CURRENT OPINION IN MICROBIOLOGY, 2004, 7 (03) :245-254

[4] Development and validation of a diagnostic microbial microarray for methanotrophs [J].

Bodrossy, L ;

Stralis-Pavese, N ;

Murrell, JC ;

Radajewski, S ;

Weilharter, A ;

Sessitsch, A .

ENVIRONMENTAL MICROBIOLOGY, 2003, 5 (07) :566-582

[5] mRNA extraction and reverse transcription-PCR protocol for detection of nifH gene expression by Azotobacter vinelandii in soil [J].

Bürgmann, H ;

Widmer, F ;

Sigler, WV ;

Zeyer, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (04) :1928-1935

[6]

Eller G, 2001, FEMS MICROBIOL LETT, V198, P91, DOI 10.1111/j.1574-6968.2001.tb10624.x

[7] Methanotrophic bacteria [J].

Hanson, RS ;

Hanson, TE .

MICROBIOLOGICAL REVIEWS, 1996, 60 (02) :439-+

[8] Quantitative detection of methanotrophs in soil by novel pmoA-targeted real-time PCR assays [J].

Kolb, S ;

Knief, C ;

Stubner, S ;

Conrad, R .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (05) :2423-2429

[9] DNA microarray applications in environmental microbiology [J].

Letowski, J ;

Brousseau, R ;

Masson, L .

ANALYTICAL LETTERS, 2003, 36 (15) :3165-3184

[10] RNA stable isotope probing, a novel means of linking microbial community function to Phylogeny [J].

Manefield, M ;

Whiteley, AS ;

Griffiths, RI ;

Bailey, MJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (11) :5367-5373

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