Characterization of new human c-myc mRNA species produced by alternative splicing

被引:6
作者
Bodescot, M [1 ]
Brison, O [1 ]
机构
[1] INST GUSTAVE ROUSSY,GENET ONCOL LAB,CNRS,URA 1967,F-94805 VILLEJUIF,FRANCE
关键词
full length cDNA cloning; clustered alternative splice sites; human carcinoma cell line;
D O I
10.1016/0378-1119(96)00464-7
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Characterization of two human c-myc cDNAs corresponding to the mRNAs 2.5 and 3.1 kb in length transcribed from PO previously demonstrated the existence of alternative acceptor sites at the end of intron 1 and intron 2, respectively [Bentley, D.L. and Groudine, M. (1986) Mol. Cell. Biol. 6, 3481-3489]. We investigated the use of these alternative acceptor sites in each c-myc mRNA species. We characterized cDNAs corresponding to c-myc mRNAs transcribed in the SW613-S human carcinoma cell line. The use of the alternative acceptor site at the end of intron 1 was demonstrated in two out of 10 cDNAs corresponding to the 3.1-kb mRNA transcribed from P0 and in three out of 10 cDNAs corresponding to the mRNAs transcribed from P1 or P2. The use of this acceptor site is therefore not restricted to the 2.5-kb mRNA transcribed from P0. The mRNAs resulting from the use of this acceptor site would encode for a variant form of the p67 polypeptide lacking one amino-acid residue. Conversely, the use of the alternative acceptor site at the end of intron 2 was not found in any of the cDNAs corresponding to the mRNAs transcribed from P0 (0/10), from P1 or P2 (0/10) and from P3 (0/10). In the course of this study, we isolated a cDNA corresponding to another new c-myc mRNA species. This mRNA is produced by alternative splicing within intron 1 and encodes only for p64.
引用
收藏
页码:115 / 120
页数:6
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