Magnetic orientation as a tool to study the initial stage of crystallization of lysozyme

被引：107

作者：

Ataka, M ^{[1
]}

Katoh, E ^{[1
]}

Wakayama, NI ^{[1
]}

机构：

[1] NATL INST MAT & CHEM RES,TSUKUBA,IBARAKI 305,JAPAN

来源：

JOURNAL OF CRYSTAL GROWTH | 1997年 / 173卷 / 3-4期

关键词：

magnetic field; magnetic orientation; sedimentation; nucleation; lysozyme; protein;

D O I：

10.1016/S0022-0248(96)00821-4

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

The tetragonal crystals of hen egg-white lysozyme align their c-axis in the direction of a magnetic field. By applying the magnetic field of 1.6 T only over some period during the whole crystallization process, it was possible to know when the crystals sedimented. It was found that crystals grew in solution, and started to sediment on reaching a critical size. We evaluated the critical size to be 1-2 mu m by changing the magnetic field strength (0.1-1.2 T) and analyzing the relation between the field strength and the proportion of magnetic orientation.

引用

页码：592 / 596

页数：5

共 6 条

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