Prolonged oxidative stress on actin

被引:75
作者
Milzani, A [1 ]
DalleDonne, I [1 ]
Colombo, R [1 ]
机构
[1] UNIV MILAN,DEPT BIOL,LAB BIOCHEM & BIOPHYS CYTOSKELETAL STRUCT,I-20133 MILAN,ITALY
关键词
oxidized-actin assembly-disassembly; altered G-actin/oxidizing media; hydrogen peroxide; diamide; DNase I inhibition;
D O I
10.1006/abbi.1996.9847
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hydrogen peroxide, coming from polymorphonuclear leukocytes, causes severe oxidative injury on actin molecules with the disarrangement of cortical actin cytoskeleton followed by plasmalemma blebbing, In this paper we demonstrate that actin oxidation does not simply develop into denaturation, but oxidative injuries on actin are specific and related to the chemical characteristics of the oxidant, Experiments on purified actin in solution have shown that actin behavior to oxidation depends on (i) the amino acidic targets of the oxidant and (ii) on the structural plasticity of the actin molecule, which differently responds to different chemical modifications, Therefore, hydrogen peroxide (that presents a broad oxidative activity) affects actin dynamics by markedly inhibiting the assembly of actin monomers, by forcing the disassembly of actin polymers, and, moreover, by affecting the interaction between oxidant-stressed actin and DNase I. Diamide (a specific thiol oxidant), in contrast, mainly lowers the actin polymerization extent, while it slightly influences the polymerization rate and results uneffective on both F-actin disassembly and actin-related; DNase I inhibition, Actin response to oxidative stresses likely depends on the ''structural connectivity in actin,'' the property allowing it to finely modulate the actin filament architecture, The potential cellular relevance of the alterations in the interaction between oxidized actin and DNase I has been briefly discussed. (C) 1997 Academic Press.
引用
收藏
页码:267 / 274
页数:8
相关论文
共 27 条
[1]   SELECTIVE ASSAY OF MONOMERIC AND FILAMENTOUS ACTIN IN CELL-EXTRACTS, USING INHIBITION OF DEOXYRIBONUCLEASE-I [J].
BLIKSTAD, I ;
MARKEY, F ;
CARLSSON, L ;
PERSSON, T ;
LINDBERG, U .
CELL, 1978, 15 (03) :935-943
[2]   REACTIONS OF SELENO- + SULFOAMINO ACIDS WITH HYDROPEROXIDES [J].
CALDWELL, KA ;
TAPPEL, AL .
BIOCHEMISTRY, 1964, 3 (11) :1643-&
[3]   STRUCTURAL CONNECTIVITY IN ACTIN - EFFECT OF C-TERMINAL MODIFICATIONS ON THE PROPERTIES OF ACTIN [J].
CROSBIE, RH ;
MILLER, C ;
CHEUNG, P ;
GOODNIGHT, T ;
MUHLRAD, A ;
REISLER, E .
BIOPHYSICAL JOURNAL, 1994, 67 (05) :1957-1964
[4]   ACTIN POLYMERIZATION AND INTRACELLULAR SOLVENT FLOW IN CELL-SURFACE BLEBBING [J].
CUNNINGHAM, CC .
JOURNAL OF CELL BIOLOGY, 1995, 129 (06) :1589-1599
[5]   ACTIN-BINDING PROTEIN REQUIREMENT FOR CORTICAL STABILITY AND EFFICIENT LOCOMOTION [J].
CUNNINGHAM, CC ;
GORLIN, JB ;
KWIATKOWSKI, DJ ;
HARTWIG, JH ;
JANMEY, PA ;
BYERS, HR ;
STOSSEL, TP .
SCIENCE, 1992, 255 (5042) :325-327
[6]   H2O2-treated actin: Assembly and polymer interactions with cross-linking proteins [J].
DalleDonne, I ;
Milzani, A ;
Colombo, R .
BIOPHYSICAL JOURNAL, 1995, 69 (06) :2710-2719
[7]   NEW INSIGHTS INTO ACTIN FILAMENT DYNAMICS [J].
EGELMAN, EH ;
ORLOVA, A .
CURRENT OPINION IN STRUCTURAL BIOLOGY, 1995, 5 (02) :172-180
[8]   POLYMERIZATION OF ACTIN AND ACTIN-LIKE SYSTEMS - EVALUATION OF THE TIME COURSE OF POLYMERIZATION IN RELATION TO THE MECHANISM [J].
FRIEDEN, C ;
GODDETTE, DW .
BIOCHEMISTRY, 1983, 22 (25) :5836-5843
[9]   ACTIN POLYMERIZATION IN CELLULAR OXIDANT INJURY [J].
HINSHAW, DB ;
BURGER, JM ;
BEALS, TF ;
ARMSTRONG, BC ;
HYSLOP, PA .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1991, 288 (02) :311-316
[10]  
HINSHAW DB, 1988, AM J PATHOL, V132, P479