Micropropagation of switchgrass by node culture

被引：34

作者：

Alexandrova, KS ^{[1
]}

Denchev, PD ^{[1
]}

Conger, BV ^{[1
]}

机构：

[1] UNIV TENNESSEE,DEPT PLANT & SOIL SCI,KNOXVILLE,TN 37901

来源：

CROP SCIENCE | 1996年 / 36卷 / 06期

关键词：

D O I：

10.2135/cropsci1996.0011183X003600060049x

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Switchgrass (Panicum virgatum L.) is naturally outcrossing; therefore, maintenance of genotype is difficult through sexual propagation. The objective of this study was to develop a micropropagation procedure for the multiplication of desired or selected genotypes. Nodal segments were surface sterilized, split Longitudinally, and placed with the cut surface on solid MS medium containing 30 g L(-1) maltose and different concentrations of 6-benzylaminopurine (BAP) as the only growth regulator. Best shoot proliferation was obtained with 12.5 mu M BAP and culture at 29 degrees C. Shoots were easily rooted on MS medium without BAP. Under optimum conditions, it is possible to produce approximately 500 plantlets from one parent plant in 12 wk.

引用

页码：1709 / 1711

页数：3

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