Alveolar macrophages and CC chemokines are increased in children with cystic fibrosis

被引:67
作者
Brennan, S. [2 ]
Sly, P. D. [1 ,2 ,3 ]
Gangell, C. L. [2 ]
Sturges, N. [2 ]
Winfield, K. [2 ]
Wikstrom, M. [2 ]
Gard, S. [2 ]
Upham, J. W. [2 ,4 ]
机构
[1] Univ Western Australia, Telethon Inst Child Hlth Res, Div Clin Sci, Perth, WA 6872, Australia
[2] Univ Western Australia, Ctr Child Hlth Res, Perth, WA 6872, Australia
[3] Princess Margaret Hosp Children, Dept Resp Med, Perth, WA, Australia
[4] Univ Queensland, Sch Med, Brisbane, Qld, Australia
[5] Murdoch Childrens Res Inst, Melbourne, Vic, Australia
基金
英国医学研究理事会;
关键词
CC chemokines; children; cystic fibrosis; macrophages; EARLY PULMONARY INFLAMMATION; NEUTROPHIL ELASTASE; YOUNG-CHILDREN; LUNG; INFANTS; CELLS; ASPERGILLOSIS; EXPRESSION; RESOLUTION; CLEARANCE;
D O I
10.1183/09031936.00178508
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Airway inflammation is an important component of cystic fibrosis (CF) lung disease. We sought to determine whether alveolar macrophages were involved in early CF lung disease. Children with CF (median age 3.1 yrs) participated in a surveillance programme that included annual bronchoalveolar lavage (BAL). Control samples were obtained from non-CF children (median age 3.1 yrs; n=24) investigated for persistent respiratory symptoms. Pulmonary infection was detected in 31% (16 out of 51) and 38% (nine out of 24) of children from the CF and non-CF groups, respectively. Alveolar macrophages in BAL were increased in CF compared with non-CF in the absence of infection (223 x 10(3) versus 85 x 10(3) cells.mL(-1); p=0.001) and were associated with elevations in the CC chemokines (macrophage inflammatory protein (MIP)-3 alpha (chemokine (C-C motif) ligand (CCL)20; 355.8 versus 46.0 pg.mL(-1); p<0.001), monocyte chemotactic protein-1 (CCL2; 263.5 versus 25.3 pg.mL(-1); p<0.001), MIP-1 alpha (CCL3; 38.2 versus 4.9 pg.mL(-1); p<0.001) and MIP-1 beta (CCL4; 326.6 versus 27.5 pg.mL(-1); p<0.001)). Total cell counts and neutrophil numbers increased in the presence of infection; however, there was no additional effect of CF. Alveolar macrophages and CC chemokines are elevated in the lungs in young children with CF even in the absence of pulmonary infection. Longitudinal studies are required to determine the clinical relevance of these findings.
引用
收藏
页码:655 / 661
页数:7
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