CYTOTOXICITY EVALUATION OF TWO ROOT CANAL SEALERS AND A COMMERCIAL CALCIUM HYDROXIDE PASTE ON THP1 CELL LINE BY TRYPAN BLUE ASSAY

被引：15

作者：

Bekner Correa, Gabriela Tavares ^{[1
]}

Costa Veranio, Gabriel Alves ^{[2
]}

Silva, Licinio Esmaeraldo ^{[3
]}

Hirata Junior, Raphael ^{[4
]}

Coil, Jeffry M. ^{[5
]}

Zaccaro Scelza, Miriam F. ^{[1
]}

机构：

[1] Univ Fed Fluminense, Dept Endodont, Niteroi, RJ, Brazil

[2] Univ Fed Rio de Janeiro, Lab Blood Marrow Transplantat, Rio De Janeiro, Brazil

[3] Univ Fed Fluminense, Math Inst, Niteroi, RJ, Brazil

[4] State Univ Rio Janeiro, Dept Microbiol & Parasitol, Rio De Janeiro, Brazil

[5] Univ British Columbia, Dept Oral Biol & Med Sci, Fac Dent, Vancouver, BC V5Z 1M9, Canada

来源：

JOURNAL OF APPLIED ORAL SCIENCE | 2009年 / 17卷 / 05期

关键词：

AH Plus; Fill Canal; L&C; Cytotoxicity; Trypan blue; FILLING MATERIALS; ENDODONTIC SEALERS; TISSUE-RESPONSE;

D O I：

10.1590/S1678-77572009000500020

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Objective: The aim of this investigation was to evaluate the cytotoxicity of two brands of root canal sealers, epoxy-resin based and zinc oxide-eugenol based, and one commercial calcium hydroxide paste on a monocyte cell line THP-1. Material and methods: Undiluted (crude extract) and diluted extracts to 10%, 1%, 0.1%, 0.01%, 0.001% and 0.0001% of the sealers were tested for cytotoxicity to THP-1 cells using the trypan blue assay. Extracts were obtained according to ISO standard. Data were analyzed statistically by the Kruskal-Wallis and Mann-Whitney tests at 5% significance level. Results: Crude extract of AH Plus and Fill Canal killed approximately 90% of THP-1 cells versus 36% of THP-1 cells killed by L&C crude extract (p<0.05). Ten-fold dilutions of L&C, Fill Canal and AH Plus killed 24, 35 and 61% of THP-1 cells (p<0.05), respectively. Dilutions lesser than 1% caused minimal cell death as compared to the control groups (p>0.05), except for L&C 1% extract. Conclusions: The results revealed that the L&C paste crude extract was less cytotoxic to THP-1 cells than AH Plus or Fill Canal crude extracts.

引用

页码：457 / 461

页数：5

共 24 条

[1]

[Anonymous], 2010, Culture of animal cells: a manual of basic technique and specialized applications

[2] In vitro cytotoxicity of a new epoxy resin root canal sealer [J].

Azar, NG ;

Heidari, M ;

Bahrami, ZS ;

Shokri, F .

JOURNAL OF ENDODONTICS, 2000, 26 (08) :462-465

[3]

BROWNE RM, 1988, INT ENDOD J, V21, P50

[4]

CAMPS J, 2003, J ENDODONT, V20, P83

[5]

da Costa AO, 1999, BIOCELL, V23, P65

[6] Cytotoxicity of new resin-, calcium hydroxide- and silicone-based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines [J].

Eldeniz, A. U. ;

Mustafa, K. ;

Orstavik, D. ;

Dahl, J. E. .

INTERNATIONAL ENDODONTIC JOURNAL, 2007, 40 (05) :329-337

[7] Cytokine pattern determines the progression of experimental periodontal disease induced by Actinobacillus actinomycetemcomitans through the modulation of MMPs, RANKL, and their physiological inhibitors [J].

Garlet, GP ;

Cardoso, CR ;

Silva, TA ;

Ferreira, BR ;

Avila-Campos, MJ ;

Cunha, FQ ;

Silva, JS .

ORAL MICROBIOLOGY AND IMMUNOLOGY, 2006, 21 (01) :12-20

[8] Effects of root-end filling materials on fibroblasts and macrophages in vitro [J].

Haglund, R ;

He, JN ;

Jarvis, J ;

Safavi, KE ;

Spångberg, LSW ;

Zhu, Q .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 2003, 95 (06) :739-745

[9]

International Organization for Standardization, 1999, 109935 ISO

[10] METHODS IN STUDY OF BIOLOGIC RESPONSES TO ENDODONTIC MATERIALS - TISSUE RESPONSE TO N2 [J].

LANGELAND, K ;

GUTTUSO, J ;

LANGELAND, LK ;

TOBON, G .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY, 1969, 27 (04) :522-+

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