A novel dermal matrix generated from burned skin as a promising substitute for deep-degree burns therapy

被引:35
作者
Yu, Guanying [1 ,2 ]
Ye, Lan [1 ]
Tan, Wei [2 ]
Zhu, Xuguo [1 ,3 ]
Li, Yaonan [1 ]
Jiang, Duyin [1 ]
机构
[1] Shandong Univ, Hosp 2, Dept Burns & Plast Surg, 247 Beiyuan St, Jinan 250033, Shandong, Peoples R China
[2] Jinan Cent Hosp, Dept Gastrointestinal Surg, Jinan 250013, Shandong, Peoples R China
[3] Peoples Hosp Rizhao, Dept Burns & Plast Surg, Rizhao 276800, Shandong, Peoples R China
基金
中国国家自然科学基金;
关键词
acellular dermal matrix; deep-degree burned dermal matrix; deep burn; cytokine; dermal graft; THERMAL-INJURY; HYPERTROPHIC SCAR; GROWTH-FACTOR; MYOFIBROBLASTIC PHENOTYPE; EXTRACELLULAR-MATRIX; PLASMA CYTOKINES; TOXIN; MECHANISMS; INDUCTION; MOUSE;
D O I
10.3892/mmr.2016.4866
中图分类号
R73 [肿瘤学];
学科分类号
100214 [肿瘤学];
摘要
The extensive skin defects induced by severe burns are dangerous and can be fatal. Currently, the most common therapy is tangential excision to remove the necrotic or denatured areas of skin, followed by skin grafting. Xenogeneic dermal substitutes, such as porcine acellular dermal matrix (ADM), are typically used to cover the burn wounds, and may accelerate wound healing. It is assumed that burned skin that still maintains partial biological activity may be recycled to construct an autologous acellular dermal matrix, termed 'deep-degree burned dermal matrix (DDBDM)'. In theory, DDBDM may avoid the histoincompatibility issues associated with foreign or xenogeneic dermal matrices, and reduce therapy costs by making full use of discarded skin. In the present study, the collagens within prepared DDBDM were thickened, disorganized and partially fractured, however, they still maintained their reticular structure and tensile strength (P<0.01). Through microarray analysis of the cytokines present in ADM and DDBDM, it was determined that the DDBDM did not produce excessive levels of harmful burn toxins. Following 4 weeks of subcutaneous implantation, ADM and DDBDM were incompletely degraded and maintained good integrity. No significant inflammatory reaction or rejection were observed, which indicated that ADM and DDBDM have good histocompatibility. Therefore, DDBDM may be a useful material for the treatment of deep-degree burns.
引用
收藏
页码:2570 / 2582
页数:13
相关论文
共 75 条
[1]
Cellular mechanisms underlying acute graft rejection: time for reassessment [J].
Alegre, Maria-Luisa ;
Florquin, Sandrine ;
Goldman, Michel .
CURRENT OPINION IN IMMUNOLOGY, 2007, 19 (05) :563-568
[2]
BURN TOXIN IN MOUSE SKIN [J].
ALLGOWER, M ;
CUENI, LB ;
STADTLER, K ;
SCHOENENBERGER, GA .
JOURNAL OF TRAUMA-INJURY INFECTION AND CRITICAL CARE, 1973, 13 (02) :95-111
[3]
Xenogeneic extracellular matrix grafts elicit a Th2-restricted immune response [J].
Allman, AJ ;
McPherson, TB ;
Badylak, SF ;
Merrill, LC ;
Kallakury, B ;
Sheehan, C ;
Raeder, RH ;
Metzger, DW .
TRANSPLANTATION, 2001, 71 (11) :1631-1640
[4]
A preliminary investigation of the reinnervation and return of sensory function in burn patients treated with INTEGRA® [J].
Anderson, James R. ;
Fear, Mark W. ;
Phillips, Jacqueline K. ;
Dawson, Linda F. ;
Wallace, Hilary ;
Wood, Fiona M. ;
Rea, Suzanne M. .
BURNS, 2011, 37 (07) :1101-1108
[5]
The extracellular matrix as a biologic scaffold material [J].
Badylak, Stephen F. .
BIOMATERIALS, 2007, 28 (25) :3587-3593
[6]
THE BIOLOGY OF CACHECTIN/TNF - A PRIMARY MEDIATOR OF THE HOST RESPONSE [J].
BEUTLER, B ;
CERAMI, A .
ANNUAL REVIEW OF IMMUNOLOGY, 1989, 7 :625-655
[7]
The role of thrombospondins 1 and 2 in the regulation of cell-matrix interactions, collagen fibril formation, and the response to injury [J].
Bornstein, P ;
Agah, A ;
Kyriakides, TR .
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2004, 36 (06) :1115-1125
[8]
Contractile skeletal muscle tissue-engineered on an acellular scaffold [J].
Borschel, GH ;
Dennis, RG ;
Kuzon, WM .
PLASTIC AND RECONSTRUCTIVE SURGERY, 2004, 113 (02) :595-602
[9]
The optimal time for early burn wound excision to reduce pro-inflammatory cytokine production in a murine burn injury model [J].
Chang, Ko-Chang ;
Ma, Hsu ;
Liao, Wen-Chieh ;
Lee, Chih-Kang ;
Lin, Chia-Yi ;
Chen, Chen-chien .
BURNS, 2010, 36 (07) :1059-1066
[10]
Interclass small leucine-rich repeat proteoglycan interactions regulate collagen fibrillogenesis and corneal stromal assembly [J].
Chen, Shoujun ;
Young, Marian F. ;
Chakravarti, Shukti ;
Birk, David E. .
MATRIX BIOLOGY, 2014, 35 :103-111