Anoikis: Roadblock to cell transplantation?

被引:125
作者
Zvibel, I
Smets, F
Soriano, H
机构
[1] Tel Aviv Sourasky Med Ctr, Gastroenterol Inst, IL-64239 Tel Aviv, Israel
[2] Northwestern Univ, Sch Med, Dept Pediat, Div Gastroenterol & Hepatol, Evanston, IL 60208 USA
[3] Childrens Mem Hosp, Siragusa Transplantat Ctr, Childrens Mem Inst Educ & Res, Chicago, IL USA
关键词
anoikis; cell transplantation; hepatocytes; apoptosis; transplantation;
D O I
10.3727/000000002783985404
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Cell therapy, in particular liver cell transplantation, holds great therapeutic potential and is partially hindered by the high rate of apoptosis during cell isolation, cryopreservation, and engraftment. Apoptosis occurring due to cell detachment from the extracellular matrix is a phenomenon termed "anoikis." The purpose of this review is to describe signaling mechanisms pertinent to anoikis in both immortalized cell lines, but particularly in primary normal epithelial cells. The mechanisms described include integrin signaling and survival molecules, caspase activation, and the role of mitochondrial proteins in anoikis. Strategies to prevent anoikis during isolation and cryopreservation of hepatocytes are discussed.
引用
收藏
页码:621 / 630
页数:10
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