Recent advances in bioluminescence resonance energy transfer technologies to study GPCR heteromerization

被引:61
作者
Ayoub, Mohammed A. [1 ,2 ]
Pfleger, Kevin D. G. [1 ,2 ,3 ]
机构
[1] Univ Western Australia, Mol Endocrinol Lab, GPCRs, Western Australian Inst Med Res, Perth, WA 6009, Australia
[2] Univ Western Australia, Med Res Ctr, Perth, WA 6009, Australia
[3] Dimerix Biosci Pty Ltd, Perth, WA 6009, Australia
基金
英国医学研究理事会;
关键词
PROTEIN-COUPLED RECEPTORS; INDUCED CONFORMATIONAL-CHANGES; BETA-ARRESTIN RECRUITMENT; LIVE CELLS; TRANSFER BRET; LIVING CELLS; BETA(2)-ADRENERGIC RECEPTOR; MELATONIN RECEPTORS; SIGNAL-TRANSDUCTION; HETERODIMERS;
D O I
10.1016/j.coph.2009.09.012
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The field of G protein-coupled receptor (GPCR) research has undergone a transformation in recent years due to the notion of heteromerization. In order to progress our understanding of the functional implications of this phenomenon, as well as its applicability across the diversity of GPCR subtypes, we need to continually look to improve the technologies we use to evaluate protein-protein interactions in as near a physiological setting as possible. The bioluminescence resonance energy transfer (BRET) technology has been intimately associated with the study of GPCR-GPCR interactions for the past ten years, and over this period, both the tools and the methods of analysis have continually evolved. In this review, we highlight recent advances in the BRET technology and focus particularly on the drive to establish the specificity of GPCR heteromers.
引用
收藏
页码:44 / 52
页数:9
相关论文
共 51 条
  • [1] Preferential formation of MT1/MT2 melatonin receptor heterodimers with distinct ligand interaction properties compared with MT2 homodimers
    Ayoub, MA
    Levoye, A
    Delagrange, P
    Jockers, R
    [J]. MOLECULAR PHARMACOLOGY, 2004, 66 (02) : 312 - 321
  • [2] Monitoring of ligand-independent dimerization and ligand-induced conformational changes of melatonin receptors in living cells by bioluminescence resonance energy transfer
    Ayoub, MA
    Couturier, C
    Lucas-Meunier, E
    Angers, S
    Fossier, P
    Bouvier, M
    Jockers, R
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (24) : 21522 - 21528
  • [3] Real-time analysis of agonist-induced activation of protease-activated receptor 1/Gαi1 protein complex measured by bioluminescence resonance energy transfer in living cells
    Ayoub, Mohammed A.
    Maurel, Damien
    Binet, Virginie
    Fink, Michel
    Prezeau, Laurent
    Ansanay, Herve
    Pin, Jean-Philippe
    [J]. MOLECULAR PHARMACOLOGY, 2007, 71 (05) : 1329 - 1340
  • [4] Hetero-oligomerization between β2-and β3-adrenergic receptors generates a β-adrenergic signaling unit with distinct functional properties
    Breit, A
    Lagacé, M
    Bouvier, M
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (27) : 28756 - 28765
  • [5] Emerging role of homo- and heterodimerization in G-protein-coupled receptor biosynthesis and maturation
    Bulenger, S
    Marullo, S
    Bouvier, M
    [J]. TRENDS IN PHARMACOLOGICAL SCIENCES, 2005, 26 (03) : 131 - 137
  • [6] Detection of heteromerization of more than two proteins by sequential BRET-FRET
    Carriba, Paulina
    Navarro, Gemma
    Ciruela, Francisco
    Ferre, Sergi
    Casado, Vicent
    Agnati, Luigi
    Cortes, Antoni
    Mallol, Josefa
    Fuxe, Kjell
    Canela, Enric I.
    Lluis, Carmen
    Franco, Rafael
    [J]. NATURE METHODS, 2008, 5 (08) : 727 - 733
  • [7] G protein-coupled receptor dimers: Functional consequences, disease states and drug targets
    Dalrymple, Matthew B.
    Pfleger, Kevin D. G.
    Eidne, Karin A.
    [J]. PHARMACOLOGY & THERAPEUTICS, 2008, 118 (03) : 359 - 371
  • [8] An improved bioluminescence resonance energy transfer strategy for imaging intracellular events in single cells and living subjects
    De, Abhijit
    Loening, Andreas Markus
    Gambhir, Sanjiv Sam
    [J]. CANCER RESEARCH, 2007, 67 (15) : 7175 - 7183
  • [9] BRET3: a red-shifted bioluminescence resonance energy transfer (BRET)-based integrated platform for imaging protein-protein interactions from single live cells and living animals
    De, Abhijit
    Ray, Pritha
    Loening, Andreas Markus
    Gambhir, Sanjiv Sam
    [J]. FASEB JOURNAL, 2009, 23 (08) : 2702 - 2709
  • [10] Building a new conceptual framework for receptor heteromers
    Ferre, Sergi
    Baler, Ruben
    Bouvier, Michel
    Caron, Marc G.
    Devi, Lakshmi A.
    Durroux, Thierry
    Fuxe, Kjell
    George, Susan R.
    Javitch, Jonathan A.
    Lohse, Martin J.
    Mackie, Ken
    Milligan, Graeme
    Pfleger, Kevin D. G.
    Pin, Jean-Philippe
    Volkow, Nora D.
    Waldhoer, Maria
    Woods, Amina S.
    Franco, Rafael
    [J]. NATURE CHEMICAL BIOLOGY, 2009, 5 (03) : 131 - 134