Lymphocyte electrotaxis in vitro and in vivo

被引：111

作者：

Lin, Francis ^{[1
,3
]}

Baldessari, Fabio ^{[2
]}

Gyenge, Christina Crenguta ^{[1
,3
]}

Sato, Tohru ^{[1
,3
]}

Chambers, Robert D. ^{[2
]}

Santiago, Juan G. ^{[2
]}

Butcher, Eugene C. ^{[1
,3
]}

机构：

[1] Stanford Univ, Sch Med, Dept Pathol, Lab Immunol & Vasc Biol, Stanford, CA 94305 USA

[2] Stanford Univ, Dept Mech Engn, Stanford, CA 94305 USA

[3] Vet Affairs Palo Alto Hlth Care Syst, Ctr Mol Biol & Med, Palo Alto, CA 94304 USA

来源：

JOURNAL OF IMMUNOLOGY | 2008年 / 181卷 / 04期

关键词：

D O I：

10.4049/jimmunol.181.4.2465

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

Electric fields are generated in vivo in a variety of physiologic and pathologic settings, including penetrating injury to epithelial barriers. An applied electric field with strength within the physiologic range can induce directional cell migration (i.e., electrotaxis) of epithelial cells, endothelial cells, fibroblasts, and neutrophils suggesting a potential role in cell positioning during wound healing. In the present study, we investigated the ability of lymphocytes to respond to applied direct current (DC) electric fields. Using a modified Transwell assay and a simple microfluidic device, we show that human PBLs migrate toward the cathode in physiologically relevant DC electric fields. Additionally, electrical stimulation activates intracellular kinase signaling pathways shared with chemotactic stimuli. Finally, video microscopic tracing of GFP-tagged immunocytes in the skin of mouse cars reveals that motile cutaneous T cells actively migrate toward the cathode of an applied DC electric field. Lymphocyte positioning within tissues can thus be manipulated by externally applied electric fields, and may be influenced by endogenous electrical potential gradients as well.

引用

页码：2465 / 2471

页数：7

共 32 条

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[2]

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