Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome

被引：38

作者：

Kuspa, A ^{[1
]}

Loomis, WF ^{[1
]}

机构：

[1] UNIV CALIF SAN DIEGO,DEPT BIOL,LA JOLLA,CA 92093

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1996年 / 93卷 / 11期

关键词：

D O I：

10.1073/pnas.93.11.5562

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

High resolution gene maps of the six chromosomes of Dictyostelium discoideum have been generated by a combination of physical mapping techniques. A set of yeast artificial chromosome clones has been ordered into overlapping arrays that cover >98% of the 34-megabase pair genome. Clones were grouped and ordered according to the genes they carried, as determined by hybridization analyses with DNA fragments from several hundred genes. Congruence of the gene order within each arrangement of clones with the gene order determined from whole genome restriction site mapping indicates that a high degree of confidence can be placed on the clone map. This clone-based description of the Dictyostelium chromosomes should be useful for the physical mapping and subcloning of new genes and should facilitate more detailed analyses of this genome.

引用

页码：5562 / 5566

页数：5

共 41 条

[1] REPLACEMENT OF THE PHOSPHOLIPID-ANCHOR IN THE CONTACT SITE-A GLYCOPROTEIN OF D-DISCOIDEUM BY A TRANSMEMBRANE REGION DOES NOT IMPEDE CELL-ADHESION BUT REDUCES RESIDENCE TIME ON THE CELL-SURFACE [J].