Role of ULK-FIP200 complex in mammalian autophagy FIP200, a counterpart of yeast Atg 17?

被引：97

作者：

Hara, Taichi ^{[1
]}

Mizushima, Noboru ^{[1
]}

机构：

[1] Tokyo Med & Dent Univ, Dept Physiol & Cell Biol, Bunkyo Ku, Tokyo 1138519, Japan

来源：

AUTOPHAGY | 2009年 / 5卷 / 01期

关键词：

ULK1; FIP200; Atg1; Atg17; isolation membrane; PAS; SACCHAROMYCES-CEREVISIAE; SERINE/THREONINE KINASE; MOLECULAR MACHINERY; AXONAL ELONGATION; PROTEIN COMPLEXES; STARVATION; IDENTIFICATION; ORGANIZATION; UNC-51; GENES;

D O I：

10.4161/auto.5.1.7180

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The yeast serine threonine kinase Atg1 appears to be a key regulator of autophagy and its kinase activity is crucial for autophagy induction. Recent reports have indicated that a mammalian Atg1 homolog, UNC-51-like kinase (ULK) 1, is required for autophagy. We found that ULK1 localizes to the autophagic isolation membrane and its kinase activity is important for autophagy induction. Furthermore, we identified a focal adhesion kinase (FAK) family interacting protein of 200 W (FIP200) as a ULK-interacting protein. FIP200 also localizes to the isolation membrane together with ULK. Using FIP200-deficient cells, we found that FIP200 is essential for autophagosome formation and the proper function of ULK. Here, we discuss the role of the ULK-FIP200 complex in autophagy and the possibility that FIP200 functions as a mammalian counterpart of Atg17.

引用

页码：85 / 87

页数：3

共 30 条

[1] SiRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy [J].

Chan, Edmond Y. W. ;

Kir, Serkan ;

Tooze, Sharon A. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (35) :25464-25474

[2] Identification of RB1CC1, a novel human gene that can induce RB1 in various human cells [J].

Chano, T ;

Ikegawa, S ;

Kontani, K ;

Okabe, H ;

Baldini, N ;

Saeki, Y .

ONCOGENE, 2002, 21 (08) :1295-1298

[3] Atg17 regulates the magnitude of the autophagic response [J].

Cheong, H ;

Yorimitsu, T ;

Reggiori, F ;

Legakis, JE ;

Wang, CW ;

Klionsky, DJ .

MOLECULAR BIOLOGY OF THE CELL, 2005, 16 (07) :3438-3453

[4] The Atg1 kinase complex is involved in the regulation of protein recruitment to initiate sequestering vesicle formation for nonspecific autophagy in Saccharomyces cerevisiae [J].

Cheong, Heesun ;

Nair, Usha ;

Geng, Jiefei ;

Klionsky, Daniel J. .

MOLECULAR BIOLOGY OF THE CELL, 2008, 19 (02) :668-681

[5] FIP200, a key signaling node to coordinately regulate various cellular processes [J].

Gan, Boyi ;

Guan, Jun-Lin .

CELLULAR SIGNALLING, 2008, 20 (05) :787-794

[6] Proteome survey reveals modularity of the yeast cell machinery [J].

Gavin, AC ;

Aloy, P ;

Grandi, P ;

Krause, R ;

Boesche, M ;

Marzioch, M ;

Rau, C ;

Jensen, LJ ;

Bastuck, S ;

Dümpelfeld, B ;

Edelmann, A ;

Heurtier, MA ;

Hoffman, V ;

Hoefert, C ;

Klein, K ;

Hudak, M ;

Michon, AM ;

Schelder, M ;

Schirle, M ;

Remor, M ;

Rudi, T ;

Hooper, S ;

Bauer, A ;

Bouwmeester, T ;

Casari, G ;

Drewes, G ;

Neubauer, G ;

Rick, JM ;

Kuster, B ;

Bork, P ;

Russell, RB ;

Superti-Furga, G .

NATURE, 2006, 440 (7084) :631-636

[7] FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells [J].

Hara, Taichi ;

Takamura, Akito ;

Kishi, Chieko ;

Iemura, Shun-ichiro ;

Natsume, Tohru ;

Guan, Jun-Lin ;

Mizushima, Noboru .

JOURNAL OF CELL BIOLOGY, 2008, 181 (03) :497-510

[8] Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry [J].

Ho, Y ;

Gruhler, A ;

Heilbut, A ;

Bader, GD ;

Moore, L ;

Adams, SL ;

Millar, A ;

Taylor, P ;

Bennett, K ;

Boutilier, K ;

Yang, LY ;

Wolting, C ;

Donaldson, I ;

Schandorff, S ;

Shewnarane, J ;

Vo, M ;

Taggart, J ;

Goudreault, M ;

Muskat, B ;

Alfarano, C ;

Dewar, D ;

Lin, Z ;

Michalickova, K ;

Willems, AR ;

Sassi, H ;

Nielsen, PA ;

Rasmussen, KJ ;

Andersen, JR ;

Johansen, LE ;

Hansen, LH ;

Jespersen, H ;

Podtelejnikov, A ;

Nielsen, E ;

Crawford, J ;

Poulsen, V ;

Sorensen, BD ;

Matthiesen, J ;

Hendrickson, RC ;

Gleeson, F ;

Pawson, T ;

Moran, MF ;

Durocher, D ;

Mann, M ;

Hogue, CWV ;

Figeys, D ;

Tyers, M .

NATURE, 2002, 415 (6868) :180-183

[9] Atg7-dependent autophagy promotes neuronal health, stress tolerance, and longevity but is dispensable for metamorphosis in Drosophila [J].

Juhasz, Gabor ;

Erdi, Balazs ;

Sass, Miklos ;

Neufeld, Thomas P. .

GENES & DEVELOPMENT, 2007, 21 (23) :3061-3066

[10] Atg17 functions in cooperation with Atg1 and Atg13 in yeast autophagy [J].

Kabeya, Y ;

Kamada, Y ;

Baba, M ;

Takikawa, H ;

Sasaki, M ;

Ohsumi, Y .

MOLECULAR BIOLOGY OF THE CELL, 2005, 16 (05) :2544-2553

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