Development and characterization of cobalt hexacyanoferrate modified carbon electrodes for electrochemical enzyme Biosensors

被引：45

作者：

Florescu, M

Brett, CMA ^{[1
]}

机构：

[1] Univ Coimbra, Dept Quim, P-3004535 Coimbra, Portugal

[2] Transilvania Univ Brasov, Dept Phys, Brasov, Romania

来源：

ANALYTICAL LETTERS | 2004年 / 37卷 / 05期

关键词：

carbon film; cobalt hexacyanoferrate; enzyme electrode; glucose; glucose oxidase;

D O I：

10.1081/AL-120030284

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Carbon film electrodes have been modified with films of cobalt hexacyanoferrate by potential cycling from solutions containing cobalt and hexacyanoferrate ions. The voltammetric characteristics of the films have been investigated in different electrolyte solutions and the properties related to insertion reactions within the crystal structure. The application of these modified electrodes as redox mediators in enzyme biosensors has been investigated using the mediated detection of hydrogen peroxide, demonstrated by the determination of glucose using glucose oxidase. Excellent detection limits in the micromolar region have been attained and the principle of measurement in real samples demonstrated by that of glucose in sweet wine.

引用

页码：871 / 886

页数：16

共 39 条

[1] CHARACTERIZATION OF MEDIATED AND NON-MEDIATED OXIDASE ENZYME-BASED GLASSY-CARBON ELECTRODES [J].