A simple in vivo approach to investigate invasive trophoblast cells

被引:11
作者
Arroyo, JA [1 ]
Konno, T [1 ]
Khalili, DC [1 ]
Soares, MJ [1 ]
机构
[1] Univ Kansas, Med Ctr, Dept Pathol & Lab Med, Inst Maternal Fetal Biol,Div Canc & Dev Biol, Kansas City, KS 66160 USA
关键词
trophoblast invasion; placentation; metrial gland; rat pregnancy;
D O I
10.1387/ijdb.051993ja
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Intrauterine trophoblast cell invasion is an essential part of hemochorial placentation. Aberrant trophoblast cell invasion has been associated with pathologies including preeclampsia and fetal growth restriction. In this study, we describe an in vivo method to assess trophoblast cell invasion using a transgenic rat model, constitutively expressing heat stable human placental alkaline phosphatase (Rosa 26 promoter driven human placental alkaline phosphatase, R26-hAP). Wild-type female Fischer 344 inbred rats were mated with hemizygous R26-hAP transgenic male Fischer 344 rats and sacrificed during the second half of pregnancy. Heat stable alkaline phosphatase (AP) activity associated with the invasive transgenic trophoblast cells was monitored in the wild-type uterine mesometrial compartment and used as an index of trophoblast cell invasion. The expression pattern of cytokeratins by invasive trophoblast cells mimicked the uterine mesometrial distribution of AP activity. Trophoblast cell invasion exhibited a gestation-dependent profile with peak invasion between days 18-20 of pregnancy. In summary, we have devised a simple in vivo method for assessing intrauterine trophoblast cell invasion. This technique should facilitate the discovery of endogenous regulatory mechanisms controlling trophoblast cell invasion and should represent an effective method of testing the impact of various environmental stressors on an essential part of hemochorial placentation.
引用
收藏
页码:977 / 980
页数:4
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