Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology

被引：26

作者：

Giglio, S ^{[1
]}

Monis, PT ^{[1
]}

Saint, CP ^{[1
]}

机构：

[1] Australian Water Qual Ctr, S Australian Water Corp, Salisbury, SA 5108, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2005年 / 71卷 / 12期

关键词：

D O I：

10.1128/AEM.71.12.8944-8948.2005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The currently accepted culture techniques for the detection of Legionella spp. in water samples (AS/NZS 3896:1998 and ISO 11731 standard methods) are slow and laborious, requiring from 7 to 14 days for a result. We describe a fully validated rapid confirmation technique that uses real-time PCR incorporating the intercalating dye SYTO9 for the direct identification of primary cultures, significantly decreasing turnaround time and allowing faster remedial action to be taken by the industry.

引用

页码：8944 / 8948

页数：5

共 19 条

[1]

[Anonymous], 1998, 11731 ISO

[2]

*ASNZS, 1998, 3896 ASNZS

[3] Legionella:: from environmental habitats to disease pathology, detection and control [J].

Atlas, RM .

ENVIRONMENTAL MICROBIOLOGY, 1999, 1 (04) :283-293

[4] Identification methods for Legionella from environmental samples [J].

Bartie, C ;

Venter, SN ;

Nel, LH .

WATER RESEARCH, 2003, 37 (06) :1362-1370

[5]

Broadbent C, 1996, NATL ENV HLTH FORUM

[6] Detection of Legionella species in respiratory specimens using PCR with sequencing confirmation [J].

Cloud, JL ;

Carroll, KC ;

Pixton, P ;

Erali, M ;

Hillyard, DR .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) :1709-1712

[7] Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR [J].

Giglio, S ;

Monis, PT ;

Saint, CP .

NUCLEIC ACIDS RESEARCH, 2003, 31 (22) :e136

[8] Nosocomial legionellosis traced to a contaminated ice machine [J].

Graman, PS ;

Quinlan, GA ;

Rank, JA .

INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 1997, 18 (09) :637-640

[9] ENZYME-LINKED IMMUNOASSAY FOR DETECTION OF PCR-AMPLIFIED DNA OF LEGIONELLAE IN BRONCHOALVEOLAR FLUID [J].

JONAS, D ;

ROSENBAUM, A ;

WEYRICH, S ;

BHAKDI, S .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (05) :1247-1252

[10]

Koide M, 2001, J Infect Chemother, V7, P224, DOI 10.1007/s101560170017

← 1 2 →