Collagens I and III in a porcine bronchial model of obliterative bronchiolitis

被引:16
作者
Alho, HS
Inkinen, KA
Salminen, US
Maasilta, PK
Taskinen, EI
Glumoff, V
Vuorio, EI
Ikonen, TS
Harjula, ALJ
机构
[1] Univ Helsinki Hosp, Dept Pulm Med, FIN-00029 Helsinki, Finland
[2] Univ Helsinki Hosp, Transplantat Lab, FIN-00029 Helsinki, Finland
[3] Univ Helsinki Hosp, Dept Surg, FIN-00029 Helsinki, Finland
[4] Univ Helsinki Hosp, Transplant Unit, Res Lab, FIN-00029 Helsinki, Finland
[5] Univ Oulu, Dept Pediat, SF-90100 Oulu, Finland
[6] Univ Oulu, Bioctr Oulu, Oulu, Finland
[7] Univ Turku, Dept Med Biochem & Mol Biol, Turku, Finland
关键词
obliterative bronchiolitis; experimental lung transplantation; collagens I and III;
D O I
10.1164/ajrccm.164.8.2011046
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
The main extracellular matrix components of the lung, type I and III collagens, were studied in chronic allograft rejection developing in a porcine heterotopic bronchial transplantation model. Specific porcine complementary DNA probes were constructed for detection of the expression of type I and III procollagen messenger RNAs in the bronchial wall structures and in the obliterative plug by in situ hybridization. In autografts, and in allografts immunosuppressed with 40-O-(2-hydroxyethyl)-rapamycin, cyclosporine A, and methylprednisolone, no histological changes of obliterative bronchiolitis (OB) developed, and the number of fibroblast-like cells expressing type I and III procollagen mRNA remained low. In nontreated allografts obliterating within 21 d, a preponderance of fibroblast-like cells showing positivity for type III procollagen mRNA existed in the obliterative plug and bronchial wall. This study shows for the first time the temporal and spatial activation of type I and III procollagen genes during the course of obliterative bronchiolitis. The number of cells expressing procollagen III mRNA increased parallel to developing obliteration and fibrosis in nontreated allografts, whereas autografts and immunosuppressed allografts exhibited no such trend. This finding suggests a positive association between type III collagen mRNA expression in fibroblast-like cells and development of obliterative bronchiolitis.
引用
收藏
页码:1519 / 1525
页数:7
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