Overproduction, purification, and characterization of recombinant aspartate semialdehyde dehydrogenase from Arabidopsis thaliana

被引：23

作者：

Paris, S ^{[1
]}

Wessel, PM ^{[1
]}

Dumas, R ^{[1
]}

机构：

[1] CNRS, INRA, Unite Mixte, Aventis CropSci,UMR 1932, F-69263 Lyon, France

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2002年 / 24卷 / 01期

关键词：

ASDH; ASADH; Arabidopsis thaliana;

D O I：

10.1006/prep.2001.1538

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In plant and microorganisms, aspartate semialdehyde dehydrogenase (ASDH) produces the branch point intermediate between the lysine and threonine/methionine pathways. In this study, we report the first cDNA cloning, purification, and characterization of a plant ASDH. The Arabidopsis thaliana ASDH is an homodimeric enzyme composed of subunits of 36 kDa. The plant enzyme exhibited a specific activity of 26 mumol NADPH oxidized min(-1) mg(-1) of protein with a K-M value for NADPH of 92 muM. ASDH showed cooperative behavior for aspartyl phosphate with a K-0.5 value of 37 muM. (C) 2002 Elsevier Science (USA).

引用

页码：99 / 104

页数：6

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