A simple, sensitive, non-stimulated photon counting system for detection of superoxide anion in whole blood

被引：70

作者：

Lu, FJ

Lin, JT

Wang, HP

Huang, WC

机构：

[1] NATL TAIWAN UNIV,COLL MED,DEPT INTERNAL MED,TAIPEI,TAIWAN

[2] NATL TAIWAN UNIV,COLL MED,DEPT EMERGENCY,TAIPEI,TAIWAN

来源：

EXPERIENTIA | 1996年 / 52卷 / 02期

关键词：

superoxide; free radical; reactive oxygen species; chemiluminescence; pancreatitis;

D O I：

10.1007/BF01923359

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A simple, sensitive, non-stimulated assay was developed to measure the superoxide anion concentration in whole blood, using an ultra-sensitive chemiluminescence (CL) analyzer and lucigenin amplification. The assay system can be performed without leukocyte isolation or stimulant administration. The blood CL levels of healthy males (362.8 +/- 337.7 counts/10 sec) were not different from those of females (335 +/- 308.7 counts/10 sec) (p = 0.64), whereas the CL levels in whole blood in patients with acute pancreatitis (2522 +/- sec) were significantly higher than those of healthy controls (p < 0.001). This assay system may be valuable in the future for quantitative measurement of reactive oxygen species in various disorders.

引用

页码：141 / 144

页数：4

共 25 条

[1] ANALYSIS OF POLYMORPHONUCLEAR LEUKOCYTE RESPIRATORY BURST ACTIVITY IN UREMIC PATIENTS USING WHOLE-BLOOD CHEMILUMINESCENCE [J].