Disruption of the mycobacterial cell entry gene of Mycobacterium bovis BCG results in a mutant that exhibits a reduced invasiveness for epithelial cells

被引：26

作者：

Flesselles, B ^{[1
]}

Anand, NN ^{[1
]}

Remani, J ^{[1
]}

Loosmore, SM ^{[1
]}

Klein, MH ^{[1
]}

机构：

[1] Pasteur Merieux Connaught Canada Res Ctr, N York, ON M2R 3T4, Canada

来源：

FEMS MICROBIOLOGY LETTERS | 1999年 / 177卷 / 02期

关键词：

bacillus Calmette-Guerin; homologous recombination; cell invasion;

D O I：

10.1111/j.1574-6968.1999.tb13738.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mycobacteria belonging to the Mycobacterium tuberculosis complex have the ability to invade and replicate in nonphagocytic cells, an event that requires the presence of bacterial surface components capable of triggering a cell response and the subsequent internalization of the microorganism. In this study, we report the sequencing of the mycobacterial cell entry gene (mce) of Mycobacterium bovis bacillus Calmette-Guerin (BCG) and the generation and characterization of a mutant BCG strain with an inactivated mce gene, by homologous recombination with double cross-over. This mutant strain does not express the mycobacterial cell entry protein (Mce) and exhibits a reduced ability to invade the non-phagocytic epithelial cell line HeLa as compared to wild-type BCG. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：237 / 242

页数：6

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