T-STAR/ETOILE: a novel relative of SAM68 that interacts with an RNA-binding protein implicated in spermatogenesis

被引:100
作者
Venables, JP
Vernet, C
Chew, L
Elliott, DJ
Cowmeadow, RB
Wu, J
Cooke, HJ
Artzt, K
Eperon, IC
机构
[1] Univ Leicester, Dept Biochem, Leicester LE1 7RH, Leics, England
[2] Univ Texas, Dept Microbiol, Inst Mol & Cellular Biol, Austin, TX 78712 USA
[3] Univ London Queen Mary & Westfield Coll, St Bartholomews & Royal London Sch Med & Dent, London EC1A 7BE, England
[4] Western Gen Hosp, MRC, Human Genet Unit, Edinburgh EH4 2XU, Midlothian, Scotland
基金
英国惠康基金;
关键词
D O I
10.1093/hmg/8.6.959
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
RBM is an RNA-binding protein encoded on the Y chromosome in mammals and is expressed only in the nuclei of male germ cells, Genetic evidence from infertile men implicates it in spermatogenesis, but its function is unknown, Of a number of potential partners for RBM identified by a yeast two-hybrid screen with testis cDNA, the most frequent isolates encoded a novel RNA-binding protein, termed T-STAR, that is closely related to SAM68, an Src-associated protein of unknown function, The mouse homologue was also cloned and designated etoile, It mapped to chromosome 15, while T-STAR mapped to the syntenic region on human chromosome 8, T-STAR/etoile is expressed primarily in the testis; in rat germ cells, the expression of both T-STAR/etoile and SAM68 is regulated during meiosis. Transfection of T-STAR/etoile fused with green fluorescent protein into HeLa cells caused an accumulation of protein in a novel compartment of the nucleus, adjacent to the nucleolus but distinct from the peri-nucleolar compartment. RBM and other hnRNP G family members are candidate downstream targets for regulation by T-STAR/ETOILE and SAM68.
引用
收藏
页码:959 / 969
页数:11
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