HITS-CLIP: panoramic views of protein-RNA regulation in living cells

被引：282

作者：

Darnell, Robert B. ^{[1
]}

机构：

[1] Rockefeller Univ, Howard Hughes Med Inst, Lab Neurooncol, New York, NY 10065 USA

来源：

WILEY INTERDISCIPLINARY REVIEWS-RNA | 2010年 / 1卷 / 02期

关键词：

MENTAL-RETARDATION PROTEIN; HETEROGENEOUS NUCLEAR-RNA; GENOME-WIDE ANALYSIS; PRE-RIBOSOMAL-RNA; BINDING PROTEIN; MESSENGER-RNA; CROSS-LINKING; IN-VIVO; POSTTRANSCRIPTIONAL REGULATION; SPLICING REGULATION;

D O I：

10.1002/wrna.31

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The study of gene regulation in cells has recently begun to shift from a period dominated by the study of transcription factor-DNA interactions to a new focus on RNA regulation. This was sparked by the still-emerging recognition of the central role for RNA in cellular complexity emanating from the RNA World hypothesis, and has been facilitated by technologic advances, in particular high throughput RNA sequencing and crosslinking methods (RNA-Seq, CLIP, and HITS-CLIP). This study will place these advances in context, and, focusing on CLIP, will explain the method, what it can be used for, and how to approach using it. Examples of the successes, limitations, and future of the technique will be discussed. (C) 2010 John Wiley & Sons, Ltd. WIREs RNA 2010 1 266-286

引用

页码：266 / 286

页数：21

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