Photochemical immobilization of proteins on microwave-synthesized photoreactive polymers

被引：32

作者：

Naqvi, A ^{[1
]}

Nahar, P ^{[1
]}

机构：

[1] Inst Genom & Integrat Biol, Delhi 110007, India

来源：

ANALYTICAL BIOCHEMISTRY | 2004年 / 327卷 / 01期

关键词：

microwave; photoreactive polymer; photoimmobilization; horseradish peroxidase; 1-fluoro-2-nitro-4-azidobenzene;

D O I：

10.1016/j.ab.2003.11.026

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a rapid and versatile procedure for the preparation of photoreactive polymers and light-induced immobilization of proteins onto such polymers. Photoreactive controlled-pore glass, silica gel, glass slide, and polystyrene microtiter plate are prepared in 40-60 s by microwave irradiation of the respective amino polymers and 1-fluoro-2-nitro-4-azidobenzene. Azido group, now part of the polymer, yields highly reactive nitrene under ultraviolet (UV) light at 365 nm. Thus, when photoreactive polymer and horseradish peroxidase or glucose oxidase are exposed to UV light, the reactive nitrene immobilizes the protein molecules in 10 to 20 min through covalent bonding. As nitrene has a property of inserting into C-H bond, the method may find potential applications for immobilization of biomolecules irrespective of their functional groups. (C) 2004 Elsevier Inc. All rights reserved.

引用

页码：68 / 73

页数：6

共 20 条

[1] Covalent immobilization of proteins onto photoactivated polystyrene microtiter plates for enzyme-linked immunosorbent assay procedures [J].