Mapping immune processes in intact tissues at cellular resolution

被引：33

作者：

Brede, Christian ^{[2
,3
]}

Friedrich, Mike ^{[4
]}

Jordan-Garrote, Ana-Laura ^{[2
,3
]}

Riedel, Simone S. ^{[2
,3
]}

Baeuerlein, Carina A. ^{[2
,3
]}

Heinze, Katrin G. ^{[4
]}

Bopp, Tobias ^{[5
]}

Schulz, Stephan ^{[6
]}

Mottok, Anja ^{[7
]}

Kiesel, Carolin ^{[3
]}

Mattenheimer, Katharina ^{[3
]}

Ritz, Miriam ^{[3
]}

von Krosigk, Viktoria

Rosenwald, Andreas ^{[7
]}

Einsele, Hermann

Negrin, Robert S. ^{[8
]}

Harms, Gregory S. ^{[4
]}

Beilhack, Andreas ^{[1
,2
,3
]}

机构：

[1] Wurzburg Univ Hosp, IZKF Res Grp, Dept Med 2, Ctr Expt Mol Med, D-97078 Wurzburg, Germany

[2] Grad Sch Life Sci, Wurzburg, Germany

[3] Univ Wurzburg, Interdisciplinary Ctr Clin Res IZKF, Wurzburg, Germany

[4] Deutsch Forsch Gemeinschaft DFG Res Ctr Expt Biom, Rudolf Virchow Ctr, Wurzburg, Germany

[5] Johannes Gutenberg Univ Mainz, Inst Immunol, Univ Med Ctr, D-6500 Mainz, Germany

[6] Charite, Inst Pathol, Berlin, Germany

[7] Univ Wurzburg, Inst Pathol, D-8700 Wurzburg, Germany

[8] Stanford Univ, Dept Med, Div Blood & Marrow Transplantat, Stanford, CA 94305 USA

来源：

JOURNAL OF CLINICAL INVESTIGATION | 2012年 / 122卷 / 12期

关键词：

VERSUS-HOST-DISEASE; MEMORY T-CELLS; MICROSCOPY; NAIVE; ULTRAMICROSCOPY; MIGRATION; ADHESION;

D O I：

10.1172/JCI65100

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Understanding the spatiotemporal changes of cellular and molecular events within an organism is crucial to elucidate the complex immune processes involved in infections, autoimmune disorders, transplantation, and neoplastic transformation and metastasis. Here we introduce a novel multicolor light sheet fluorescence microscopy (LSFM) approach for deciphering immune processes in large tissue specimens on a single-cell level in 3 dimensions. We combined and optimized antibody penetration, tissue clearing, and triple-color illumination to create a method for analyzing intact mouse and human tissues. This approach allowed us to successfully quantify changes in expression patterns of mucosal vascular addressin cell adhesion molecule-1 (MAdCAM-1) and T cell responses in Peyer's patches following stimulation of the immune system. In addition, we employed LSFM to map individual T cell subsets after hematopoietic cell transplantation and detected rare cellular events. Thus, we present a versatile imaging technology that should be highly beneficial in biomedical research.

引用

页码：4439 / 4446

页数：8

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