Steady-state levels of aquaporin 1 mRNA expression are increased in idiopathic polyhydramnios

被引:60
作者
Mann, SE
Dvorak, N
Gilbert, H
Taylor, RN
机构
[1] Maricopa Cty Gen Hosp, Phoenix Integrated Residency Obstet & Gynecol, Phoenix, AZ 85008 USA
[2] Univ Calif San Francisco, Sch Med, Dept Obstet Gynecol & Reprod Sci, San Francisco, CA USA
关键词
aquaporin; 1; fetal membranes; polyhydramnios; intramembranous pathway;
D O I
10.1016/j.ajog.2005.07.004
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: Polyhydramnios is a condition associated with significant perinatal morbidity. While the exact pathophysiology of this condition is unknown in the absence of obvious anatomic or organic etiologies, impaired intramembranous water transport has been shown. Previous studies from our laboratory have shown that the water channel aquaporin 1 (AQP1) is expressed in human fetal membranes from term pregnancies with normal amniotic fluid (AF) volume. Therefore, we hypothesized that in pregnancies with idiopathic polyhydramnios, AQP1 expression might be reduced in fetal membranes from pregnancies with this AF volume disorder. Study design: Placentas were collected from women at term (37-40 weeks) who presented with either polyhydramnios (amniotic fluid index [AFI] > 24.0 cm) or normal AF volume (AFI 5.0-23.9 cm). Immediately after delivery, the membranes (amnion and chorion) directly overlying the placenta and the free-floating reflected membranes were sampled (total of 4 samples from each placenta). RNA was isolated from each sample and expression was quantified using realtime reverse transcriptase polymerase chain reaction (PCR) and relative quantification of gene expression. Results: Relative to pregnancies with normal AF volume, there was an increase in expression of the water channel AQP1 in all regions of the fetal membranes. The greatest increase (33-fold) was seen in the reflected amnion. Conclusion: AQP1 expression is increased in polyhydramnios. This finding suggests that alterations in AQP1 expression may be a compensatory response to and not a cause of idiopathic polyhydramnios. We speculate that therapies focused on regulating AQP1 expression may be useful for treating this condition. (c) 2006 Mosby, Inc. All rights reserved.
引用
收藏
页码:884 / 887
页数:4
相关论文
共 19 条
[1]   Comparison of amniotic and intramembranous unidirectional permeabilities in late-gestation sheep [J].
Adams, EA ;
Choi, HM ;
Cheung, CY ;
Brace, RA .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2005, 193 (01) :247-255
[2]  
ANDERSON DF, 1990, J PHYSIOL-LONDON, V431, P1, DOI 10.1113/jphysiol.1990.sp018317
[3]   EXTRAPLACENTAL TRANSFER OF WATER IN THE SHEEP [J].
ANDERSON, DF ;
FABER, JJ ;
PARKS, CM .
JOURNAL OF PHYSIOLOGY-LONDON, 1988, 406 :75-84
[4]   Regulation of amniotic fluid volume: intramembranous solute and volume fluxes in late gestation fetal sheep [J].
Brace, RA ;
Vermin, ML ;
Huijssoon, E .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2004, 191 (03) :837-846
[5]   CURRENT TOPIC - PROGRESS TOWARD UNDERSTANDING THE REGULATION OF AMNIOTIC-FLUID VOLUME - WATER AND SOLUTE FLUXES IN AND THROUGH THE FETAL MEMBRANES [J].
BRACE, RA .
PLACENTA, 1995, 16 (01) :1-18
[6]   Water channel proteins AQP3 and AQP9 are present in syncytiotrophoblast of human term placenta [J].
Damiano, A ;
Zotta, E ;
Goldstein, J ;
Reisin, I ;
Ibarra, C .
PLACENTA, 2001, 22 (8-9) :776-781
[7]   Regulation of amniotic fluid volume by intramembranous absorption in sheep: Role of passive permeability and vascular endothelial growth factor [J].
Daneshmand, SS ;
Cheung, CY ;
Brace, RA .
AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2003, 188 (03) :786-793
[8]  
GILBERT WM, 1993, SEMIN PERINATOL, V17, P150
[9]  
GILBERT WM, 1989, OBSTET GYNECOL, V74, P748
[10]  
HILL LM, 1987, OBSTET GYNECOL, V69, P21