VEGF-Mediated Fusion in the Generation of Uniluminal Vascular Spheroids

被引:37
作者
Gentile, Carmine [1 ]
Fleming, Paul A. [1 ]
Mironov, Vladimir [1 ]
Argraves, Kelley M. [1 ]
Argraves, W. Scott [1 ]
Drake, Christopher J. [1 ]
机构
[1] Med Univ S Carolina, Dept Cell Biol & Anat, Charleston, SC 29425 USA
关键词
VEGF; vascular fusion; blood vessel; micro-tissue; spheroid; endothelial cell; smooth muscle cell;
D O I
10.1002/dvdy.21720
中图分类号
R602 [外科病理学、解剖学]; R32 [人体形态学];
学科分类号
100101 ;
摘要
Embryonic mouse allantoic tissue (E8.5) was cultured in hanging drops to generate a three-dimensional vascular micro-tissue. The resulting tissue spheroids had an inner network of small diameter vessels expressing platelet endothelial cell adhesion molecule-1 (PECAM-1) and an outer layer of cells expressing SM alpha A, SM22-alpha, and SM-MHC. In a subsequent phase of culture, the fusion-promoting activity of vascular endothelial growth factor (VEGF) was used to transform the inner network of small diameter endothelial tubes into a contiguous layer of cells expressing PECAM-1, CD34, and VE-cadherin that circumscribed a central lumen-like cavity. The blood vessel-like character of the VEGF-treated spheroids was further demonstrated by their physiologically relevant vasodilatory and contractile responses, including contraction induced by KCl and relaxation stimulated by high-density lipoproteins and acetylcholine-induced nitric oxide production. Developmental Dynamics 237:2918-2925, 2008. (c) 2008 Wiley-Liss, Inc.
引用
收藏
页码:2918 / 2925
页数:8
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