Evidence for cardiac sodium-calcium exchanger association with caveolin-3

被引：52

作者：

Bossuyt, J

Taylor, BE

James-Kracke, M

Hale, CC ^{[1
]}

机构：

[1] Univ Missouri, Dalton Cardiovasc Res Ctr, Columbia, MO 65211 USA

[2] Univ Missouri, Dept Biomed Sci, Columbia, MO 65211 USA

[3] Univ Missouri, Dept Pharmacol, Columbia, MO 65211 USA

来源：

FEBS LETTERS | 2002年 / 511卷 / 1-3期

关键词：

caveola; sarcolemma; cardiac Na+-Ca+ exchange;

D O I：

10.1016/S0014-5793(01)03323-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of cardiac Na+-Ca2+ exchange (NCX1) with caveolin proteins was investigated in sarcolemmal vesicles. Western blots of sarcolemmal vesicles revealed the presence of caveolin-1, -2, and -3. NCX1 co-fractionated more closely with caveolin-3 than caveolin-1 on sucrose density gradients. NCX1 has five possible caveolin-binding motifs and NCX1 co-precipitated specifically with caveolin-3. Molecular sieve column chromatography indicated that this co-precipitation was not due to incomplete solubilization of lipid raft microdomains. Cholesterol chelation in vesicles decreased NCX1 transport activity and caveolin-3 co-precipitation. NCX1 may play a role in caveolar transmembrane signaling in addition to its role in excitation-contraction coupling. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：113 / 117

页数：5

共 17 条

[1] Evidence for functional relevance of an enhanced expression of the Na+-Ca2+ exchanger in failing human myocardium [J].