Detection of morphine in ppb range by using SPR (surface-plasmon-resonance) immunosensor

被引：51

作者：

Miura, N ^{[1
]}

Ogata, K ^{[1
]}

Sakai, G ^{[1
]}

Uda, T ^{[1
]}

Yamazoe, N ^{[1
]}

机构：

[1] HIROSHIMA PREFECTURAL UNIV, DEPT BIORESOURCES, SHOBARA, HIROSHIMA 727, JAPAN

来源：

CHEMISTRY LETTERS | 1997年 / 08期

关键词：

D O I：

10.1246/cl.1997.713

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Highly sensitive detection of morphine (MO) in ppb range was realized by using an immunosensor based on surfaceplasmon-resonance (SPR) phenomena. The addition of MO into the antibody solution was found to reduce the incident angle shift of the SPR sensor system sharply because of the inhibition effect of MO. Based on this inhibiting principle, the present sensor could detect MO very sensitively in the concentration range of 0.1 - 10 ng/cm(3) (ppb).

引用

页码：713 / 714

页数：2

共 5 条

[1] COMPETITIVE AMPEROMETRIC MORPHINE SENSOR-BASED ON AN AGAROSE IMMOBILIZED MOLECULARLY IMPRINTED POLYMER [J].

KRIZ, D ;

MOSBACH, K .

ANALYTICA CHIMICA ACTA, 1995, 300 (1-3) :71-75

[2] Sensing fatty acid binding protein with planar and fiber-optical surface plasmon resonance spectroscopy devices [J].

Kunz, U ;

Katerkamp, A ;

Reinhard, R ;

Spener, F ;

Cammann, K .

SENSORS AND ACTUATORS B-CHEMICAL, 1996, 32 (02) :149-155

[3] BIOANALYSIS WITH SURFACE-PLASMON RESONANCE [J].

LOFAS, S ;

MALMQVIST, M ;

RONNBERG, I ;

STENBERG, E ;

LIEDBERG, B ;

LUNDSTROM, I .

SENSORS AND ACTUATORS B-CHEMICAL, 1991, 5 (1-4) :79-84

[4] PIEZOELECTRIC CRYSTAL IMMUNOSENSOR FOR SENSITIVE DETECTION OF METHAMPHETAMINE (STIMULANT DRUG) IN HUMAN URINE [J].

MIURA, N ;

HIGOBASHI, H ;

SAKAI, G ;

TAKEYASU, A ;

UDA, T ;

YAMAZOE, N .

SENSORS AND ACTUATORS B-CHEMICAL, 1993, 13 (1-3) :188-191

[5] CHARACTERIZATION OF MORPHINE-SPECIFIC MONOCLONAL-ANTIBODIES SHOWING MINIMAL CROSS-REACTIVITY WITH CODEINE [J].

USAGAWA, T ;

ITOH, Y ;

HIFUMI, E ;

TAKEYASU, A ;

NAKAHARA, Y ;

UDA, T .

JOURNAL OF IMMUNOLOGICAL METHODS, 1993, 157 (1-2) :143-148

← 1 →