Comet assay of cumulus cells and spermatozoa DNA status, and the relationship to oocyte fertilization and embryo quality following ICSI

被引：21

作者：

Abu-Hassan, D ^{[1
]}

Koester, F ^{[1
]}

Shoepper, B ^{[1
]}

Schultze-Mosgau, A ^{[1
]}

Asimakopoulos, B ^{[1
]}

Diedrich, K ^{[1
]}

Al-Hasani, S ^{[1
]}

机构：

[1] Med Univ Schleswig Holstein, Womens Hosp, Dept Assisted Reprod, D-23538 Lubeck, Germany

来源：

REPRODUCTIVE BIOMEDICINE ONLINE | 2006年 / 12卷 / 04期

关键词：

comet assay; DNA fragmentation; embryo quality; ICSI results;

D O I：

10.1016/S1472-6483(10)61997-9

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

It has been postulated that apoptosis may affect Cumulus cell and sperm DNA integrity, and therefore influence the Outcome of assisted reproductive techniques. This study investigates apoptotic levels in both Cumulus cells and spermatozoa, and their relationship with fertilization and embryo quality after intracytoplasmic sperm injection (ICSI). The neutral comet assay was performed oil cumulus cells and semen samples from 55 couples with male factor infertility undergoing ICSI treatment. Cells were fixed in agarose on comet assay slides, lysed in a neutral buffer and submitted to electrophoresis. The cells were stained with SYBR green fluorescent dye, which binds to double-stranded DNA and upon excitation emits light. Analysis showed that there was no correlation between apoptosis levels and the outcome of ICSI (fertilization and embryo quality).

引用

页码：447 / 452

页数：6

共 24 条

[1] MINI-SWIM-UP - A NEW TECHNIQUE OF SPERM PREPARATION FOR INTRACYTOPLASMIC SPERM INJECTION [J].

ALHASANI, S ;

KUPKER, W ;

BASCHAT, AA ;

STURM, R ;

BAUER, O ;

DIEDRICH, C ;

DIEDRICH, K .

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 1995, 12 (07) :428-433

[2] Chromatin packaging and morphology in ejaculated human spermatozoa: evidence of hidden anomalies in normal spermatozoa [J].

Bianchi, Patrizia Grace ;

Manicardi, Gian Carlo ;

Urner, Francoise ;

Campana, Aldo ;

Sakkas, Denny .

MOLECULAR HUMAN REPRODUCTION, 1996, 2 (03) :139-144

[3]

Cayli S, 2003, Reprod Biomed Online, V7, P462

[4] Assessment of DNA integrity and morphology of ejaculated spermatozoa from fertile and infertile men before and after cryopreservation [J].

Donnelly, ET ;

Steele, EK ;

McClure, N ;

Lewis, SEM .

HUMAN REPRODUCTION, 2001, 16 (06) :1191-1199

[5] Reliability of the comet assay in cryopreserved human sperm [J].

Duty, SM ;

Singh, NP ;

Ryan, L ;

Chen, Z ;

Lewis, C ;

Huang, T ;

Hauser, R .

HUMAN REPRODUCTION, 2002, 17 (05) :1274-1280

[6]

FINDIKLI A, 2003, REPROD BIOMED ONLINE, V8, P196

[7] Apoptosis in human cumulus cells in relation to maturation stage and cleavage of the corresponding oocyte [J].

Host, E ;

Mikkelsen, AL ;

Lindenberg, S ;

Smidt-Jensen, S .

ACTA OBSTETRICIA ET GYNECOLOGICA SCANDINAVICA, 2000, 79 (11) :936-940

[8] Human cumulus granulosa cell gene expression: a predictor of fertilization and embryo selection in women undergoing IVF [J].

McKenzie, LJ ;

Pangas, SA ;

Carson, SA ;

Kovanci, E ;

Cisneros, P ;

Buster, JE ;

Amato, P ;

Matzuk, MM .

HUMAN REPRODUCTION, 2004, 19 (12) :2869-2874

[9] The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development [J].

Morris, ID ;

Ilott, S ;

Dixon, L ;

Brison, DR .

HUMAN REPRODUCTION, 2002, 17 (04) :990-998

[10] High levels of apoptosis in ejaculated spermatozoa from infertile men. [J].

Moustafa, MH ;

Saleh, RA ;

Oder, M ;

Thomas, AJ ;

Abdel-Hafez, MA ;

Agarwal, A .

FERTILITY AND STERILITY, 2002, 78 (03) :S106-S107

← 1 2 3 →