Nuclear localization and interaction of RolB with plant 14-3-3 proteins correlates with induction of adventitious roots by the oncogene rolB

被引：53

作者：

Moriuchi, H

Okamoto, C

Nishihama, R

Yamashita, I

Machida, Y

Tanaka, N ^{[1
]}

机构：

[1] Hiroshima Univ, Ctr Gene Sci, Hiroshima 7398527, Japan

[2] Nagoya Univ, Grad Sch Sci, Div Biol Sci, Chikusa Ku, Nagoya, Aichi 4648602, Japan

来源：

PLANT JOURNAL | 2004年 / 38卷 / 02期

关键词：

rolB; 14-3-3; protein-protein interaction; adventitious root induction;

D O I：

10.1111/j.1365-313X.2004.02041.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The rooting-locus gene B (rolB) on the T-DNA of the root-inducing (Ri) plasmid in Agrobacterium rhizogenes is responsible for the induction of transformed adventitious roots, although the root induction mechanism is unknown. We report here that the RolB protein of pRi1724 (1724RolB) is associated with (N) under bar icotiana (t) under bar abacum (14-3-3) under bar -like protein (omega II) under bar (Nt14-3-3 omegaII) in tobacco bright yellow (BY)-2 cells. Nt14-3-3 omegaII directly interacts with 1724RolB protein. Green fluorescent protein (GFP)-fused 1724RolB is localized to the nucleus. GFP-fused mutant 1724RolB proteins having a deletion or amino acid substitution are unable to interact with Nt14-3-3 omegaII and also show impaired nuclear localization. Moreover, these 1724RolB mutants show decreased capacity for adventitious root induction. These results suggest that adventitious root induction by 1724RolB protein correlates with its interaction with Nt14-3-3 omegaII and the nuclear localization of 1724RolB protein.

引用

页码：260 / 275

页数：16

共 73 条

[1] 14-3-3-ALPHA AND 14-3-3-DELTA ARE THE PHOSPHORYLATED FORMS OF RAF-ACTIVATING 14-3-3-BETA AND 14-3-3-ZETA - IN-VIVO STOICHIOMETRIC PHOSPHORYLATION IN BRAIN AT A SER-PRO-GLU-LYS MOTIF [J].