Effect of mobile phase acidic additives on enantioselectivity for phenylalanine analogs

被引：60

作者：

Ye, YK ^{[1
]}

Stringham, RW ^{[1
]}

机构：

[1] Dupont Merck Pharmaceut Co, Chem Proc Res & Dev, Deepwater, NJ 08023 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 927卷 / 1-2期

关键词：

enantiomer separation; mobile phase composition; phenylalanine analogues; acidic additives;

D O I：

10.1016/S0021-9673(01)01060-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of acidic mobile phase additives allows the chiral separation of underivatized phenylalanine analogs on a common amylosic column. In addition to decreasing retention and band-broadening arising from non-ideal interactions, acidic additives may also increase selectivity. This appears to be due to the minimization of non-selective binding in the recognition site. Effects of the additives are related to additive pK(a) and size. Ethanesulfonic acid was typically the most effective additive with trifluoroacetic acid being one of the least effective. (C) DuPont Pharmaceutical Company. Published by Elsevier Science B.V. All rights reserved.

引用

页码：47 / 52

页数：6

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